DTX3L Double Nickase Plasmid (h): sc-403953-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DTX3L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DTX3L Double Nickase Plasmid (h) and DTX3L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DTX3L. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DTX3L Antibody (D-10): sc-514776
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DTX3L Double Nickase Plasmid (h) | sc-403953-NIC | 20 µg | $410.00 | |||
DTX3L Double Nickase Plasmid (h2) | sc-403953-NIC-2 | 20 µg | $410.00 |
DTX3L (deltex E3 ubiquitin ligase 3L) encodes an E3 ubiquitin ligase that cooperates with PARP9 to regulate protein ubiquitination in response to interferon signaling and cellular stress. Through modulation of ubiquitin-dependent protein turnover and DNA damage-associated processes, DTX3L influences innate immune pathways, chromatin-linked signaling, and maintenance of genome integrity. Its activity has been connected to regulation of inflammatory transcriptional programs and responses to viral infection, making it relevant to studies of immune homeostasis. Altered DTX3L expression or pathway engagement has been reported across multiple disease contexts, including cancer-associated signaling and immune dysregulation, supporting mechanistic investigation in human cell models.
DTX3L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DTX3L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DTX3L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DTX3L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DTX3L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.