DR1 Antibody (D-1): sc-515083
- DR1 Antibody (D-1) is a mouse monoclonal IgG1 κ DR1 antibody provided at 200 µg/ml
- raised against amino acids 1-176 representing full length DR1 of human origin
- DR1 Antibody (D-1) is recommended for detection of DR1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DR1 Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-515083 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for DR1 Antibody (D-1) for WB applications. These reagents are now offered in bundles with DR1 Antibody (D-1) (see ordering information below).
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SEE ALSO...
DR1 Antibody (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects DR1 in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DR1 (D-1) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DR1, also known as down-regulator of transcription 1 or NC2β (negative cofactor 2 subunit β), plays a crucial role in regulating transcription by RNA polymerase II. DR1 is primarily located in the nucleus, where DR1 associates with the TFIID complex, essential for transcription initiation. An N-terminal histone fold motif in DR1 enables formation of a heterodimer with NC2α (DRAP1), creating the NC2 complex that modulates transcriptional activity. This complex acts as both a repressor and activator, depending on transcriptional machinery context. NC2 represses TATA-dependent transcription while enhancing DPE-dependent transcription, influencing gene expression in a nuanced manner. Post-translational modifications, including phosphorylation, regulate NC2 complex activity by affecting DR1 interactions with other transcription factors and overall gene regulation function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DR1 Antibody (D-1) References:
- The NC2 alpha and beta subunits play different roles in vivo. | Creton, S., et al. 2002. Genes Dev. 16: 3265-76. PMID: 12502746
- The DPE, a core promoter element for transcription by RNA polymerase II. | Kadonaga, JT. 2002. Exp Mol Med. 34: 259-64. PMID: 12515390
- DR1-like element in human topoisomerase IIalpha gene involved in enhancement of etoposide-induced apoptosis by PPARgamma ligand. | Kanbe, E., et al. 2003. Exp Hematol. 31: 300-8. PMID: 12691917
- NC2alpha interacts with BTAF1 and stimulates its ATP-dependent association with TATA-binding protein. | Klejman, MP., et al. 2004. Mol Cell Biol. 24: 10072-82. PMID: 15509807
- Efficient binding of NC2.TATA-binding protein to DNA in the absence of TATA. | Gilfillan, S., et al. 2005. J Biol Chem. 280: 6222-30. PMID: 15574413
- Mutational analysis of BTAF1-TBP interaction: BTAF1 can rescue DNA-binding defective TBP mutants. | Klejman, MP., et al. 2005. Nucleic Acids Res. 33: 5426-36. PMID: 16179647
- Global distribution of negative cofactor 2 subunit-alpha on human promoters. | Albert, TK., et al. 2007. Proc Natl Acad Sci U S A. 104: 10000-5. PMID: 17548813
- The initiator core promoter element antagonizes repression of TATA-directed transcription by negative cofactor NC2. | Malecová, B., et al. 2007. J Biol Chem. 282: 24767-76. PMID: 17584739
- The dual control of TFIIB recruitment by NC2 is gene specific. | Masson, P., et al. 2008. Nucleic Acids Res. 36: 539-49. PMID: 18048413
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DR1 Antibody (D-1) | sc-515083 | 200 µg/ml | $316.00 | |||
DR1 Antibody (D-1): m-IgG Fc BP-HRP Bundle | sc-527980 | 200 µg Ab; 10 µg BP | $354.00 | |||
DR1 Antibody (D-1): m-IgG1 BP-HRP Bundle | sc-533353 | 200 µg Ab; 20 µg BP | $354.00 | |||
DR1 Antibody (D-1) X | sc-515083 X | 200 µg/0.1 ml | $316.00 | |||
DR1 Antibody (D-1) AC | sc-515083 AC | 500 µg/ml, 25% agarose | $416.00 | |||
DR1 Antibody (D-1) HRP | sc-515083 HRP | 200 µg/ml | $316.00 | |||
DR1 Antibody (D-1) FITC | sc-515083 FITC | 200 µg/ml | $330.00 | |||
DR1 Antibody (D-1) PE | sc-515083 PE | 200 µg/ml | $343.00 | |||
DR1 Antibody (D-1) Alexa Fluor® 488 | sc-515083 AF488 | 200 µg/ml | $357.00 | |||
DR1 Antibody (D-1) Alexa Fluor® 546 | sc-515083 AF546 | 200 µg/ml | $357.00 | |||
DR1 Antibody (D-1) Alexa Fluor® 594 | sc-515083 AF594 | 200 µg/ml | $357.00 | |||
DR1 Antibody (D-1) Alexa Fluor® 647 | sc-515083 AF647 | 200 µg/ml | $357.00 | |||
DR1 Antibody (D-1) Alexa Fluor® 680 | sc-515083 AF680 | 200 µg/ml | $357.00 | |||
DR1 Antibody (D-1) Alexa Fluor® 790 | sc-515083 AF790 | 200 µg/ml | $357.00 |