DPEP1 CRISPR/Cas9 KO Plasmid (h): sc-407451
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DPEP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DPEP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DPEP1 CRISPR/Cas9 KO Plasmid (h) | sc-407451 | 20 µg | $397.00 |
Overview
DPEP1 (dipeptidase 1) encodes a membrane-anchored zinc metalloprotease predominantly localized to the apical surface of epithelial cells, where it hydrolyzes a range of dipeptides and glutathione-conjugated metabolites. Through its enzymatic activity, DPEP1 contributes to peptide turnover and xenobiotic metabolism, intersecting with glutathione-dependent detoxification processes and epithelial transport functions. Expression changes in DPEP1 have been reported in multiple epithelial-derived malignancies and inflammatory contexts, linking this enzyme to remodeling of the pericellular microenvironment and altered metabolic handling. As an ectoenzyme with defined catalytic motifs, DPEP1 serves as a tractable node for studying membrane protease biology, metabolic stress responses, and epithelial differentiation states.
DPEP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DPEP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DPEP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DPEP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DPEP1 protein expression.
This CRISPR knockout system enables efficient generation of DPEP1-deficient cell models for investigation of DPEP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DPEP1 exon(s) critical for DPEP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DPEP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DPEP1 CRISPR/Cas9 KO Plasmid (h) and DPEP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DPEP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DPEP1 HDR Plasmid (h) and DPEP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DPEP1 homology arms to support homology-directed repair at defined DPEP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.