Dok-1 Antibody (A-3): sc-6929

Dok-1 Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Dok-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Dok-1 (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dok-1 plays a crucial role in cellular signaling pathways, particularly in the regulation of cell growth and differentiation, by acting as a docking protein that interacts with various signaling molecules. Dok-1′s ability to associate with the Ras GTPase-activating protein (Ras GAP) upon tyrosine phosphorylation is significant, as this association helps modulate the activity of Ras, a key player in cell proliferation and survival. Furthermore, Dok-1 is implicated in the signaling pathways activated by several growth factors, including platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), insulin, and insulin-like growth factor (IGF), highlighting Dok-1′s importance in both normal physiology and disease states, such as chronic myelogenous leukemia, where Dok-1 is a substrate of the constitutive tyrosine kinase activity of the p210 Bcr-Abl fusion protein. Additionally, Dok-1, along with other tyrosine kinase substrates like IRS-1 and Cas, contains multiple tyrosine residues and putative SH2 binding sites, which are essential for Dok-1′s function in signal transduction.

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Dok-1 Antibody (A-3) References:

1. Dok-1 and Dok-2 deficiency induces osteopenia via activation of osteoclasts.  |  Kawamata, A., et al. 2011. J Cell Physiol. 226: 3087-93. PMID: 21732353
2. Dok-1 negatively regulates platelet integrin αIIbβ3 outside-in signalling and inhibits thrombosis in mice.  |  Niki, M., et al. 2016. Thromb Haemost. 115: 969-78. PMID: 26790499
3. Dok-1 and Dok-2 Regulate the Formation of Memory CD8+ T Cells.  |  Laroche-Lefebvre, C., et al. 2016. J Immunol. 197: 3618-3627. PMID: 27664281
4. Dok-1 regulates mast cell degranulation negatively through inhibiting calcium-dependent F-actin disassembly.  |  Du, H., et al. 2022. Clin Immunol. 238: 109008. PMID: 35421591
5. Evidence that SH2 domains promote processive phosphorylation by protein-tyrosine kinases.  |  Mayer, BJ., et al. 1995. Curr Biol. 5: 296-305. PMID: 7780740
6. Vascular endothelial cell growth factor promotes tyrosine phosphorylation of mediators of signal transduction that contain SH2 domains. Association with endothelial cell proliferation.  |  Guo, D., et al. 1995. J Biol Chem. 270: 6729-33. PMID: 7896817
7. The IRS-1 signaling system.  |  Myers, MG., et al. 1994. Trends Biochem Sci. 19: 289-93. PMID: 8048169
8. A 62-kilodalton tyrosine phosphoprotein constitutively present in primary chronic phase chronic myelogenous leukemia enriched lineage negative blast populations.  |  Wisniewski, D., et al. 1994. Leukemia. 8: 688-93. PMID: 8152267
9. A Grb2-associated docking protein in EGF- and insulin-receptor signalling.  |  Holgado-Madruga, M., et al. 1996. Nature. 379: 560-4. PMID: 8596638
10. p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells.  |  Carpino, N., et al. 1997. Cell. 88: 197-204. PMID: 9008160
11. Identification of the Abl- and rasGAP-associated 62 kDa protein as a docking protein, Dok.  |  Yamanashi, Y. and Baltimore, D. 1997. Cell. 88: 205-11. PMID: 9008161
12. Molecular cloning and characterization of p56dok-2 defines a new family of RasGAP-binding proteins.  |  Di Cristofano, A., et al. 1998. J Biol Chem. 273: 4827-30. PMID: 9478921

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dok-1 Antibody (A-3) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-Dok-1 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dok-1 Antibody (A-3) Specifications

Alternate Product Name: Dok-1

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-276

Epitope Target: Dok-1

Epitope Species: mouse

Product Reactivity/Detection: Dok-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for Dok-1 Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin