Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DOCK 8 CRISPR/Cas9 KO Plasmid (h): sc-401727
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DOCK 8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DOCK 8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DOCK 8 Antibody (G-2): sc-376911
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DOCK 8 CRISPR/Cas9 KO Plasmid (h) | sc-401727 | 20 µg | $397.00 |
Overview
DOCK8 encodes a guanine nucleotide exchange factor that activates Rho-family GTPases, coordinating actin cytoskeleton remodeling required for immune cell migration, adhesion, and formation of the immunological synapse. DOCK 8 supports lymphocyte survival and signaling, influencing processes such as chemotaxis, antigen-driven activation, and cytotoxic effector function. Disruption of DOCK8 is linked to primary immunodeficiency phenotypes characterized by impaired antiviral responses, allergic inflammation, and increased susceptibility to malignancy, underscoring its central role in immune homeostasis. In mechanistic studies, DOCK8 is frequently examined in pathways controlling cytoskeletal dynamics, cell polarity, and immune synapse stability across T cells, B cells, NK cells, and dendritic cells.
DOCK 8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DOCK8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DOCK8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DOCK8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DOCK 8 protein expression.
This CRISPR knockout system enables efficient generation of DOCK8-deficient cell models for investigation of DOCK 8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DOCK8 exon(s) critical for DOCK 8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DOCK8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DOCK 8 CRISPR/Cas9 KO Plasmid (h) and DOCK 8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DOCK8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DOCK 8 HDR Plasmid (h) and DOCK 8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DOCK8 homology arms to support homology-directed repair at defined DOCK8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.