DOCK 2 Antibody (E-7): sc-365242

DOCK 2 Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects DOCK 2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DOCK 2 Antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The DOCK2 gene encodes dedicator of cytokinesis 2 (DOCK 2), a crucial protein that plays a significant role in lymphocyte chemotaxis, which is essential for the immune response. DOCK 2 is primarily located in the cytoplasm and is involved in the cytoskeletal rearrangements necessary for lymphocyte migration in response to chemokines. This peripheral membrane protein activates Rac 1 and Rac 2 small GTPases, functioning as a guanine nucleotide exchange factor (GEF) that facilitates the exchange of GDP for GTP. Additionally, DOCK 2 is implicated in the transcriptional activation of interleukin-2 through Rac 2 activation. Structurally, DOCK 2 contains one DHR-1 (CZH-1) domain, one DHR-2 (CZH-2) domain, and one SH3 domain, with the DHR-2 domain believed to mediate GEF activity. DOCK 2 co-localizes with F-Actin and exhibits expression in various human tissues, with the highest levels found in peripheral blood leukocytes, thymus, spleen, and liver, highlighting its importance in immune system function and cellular signaling pathways.

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DOCK 2 Antibody (E-7) References:

1. Haematopoietic cell-specific CDM family protein DOCK2 is essential for lymphocyte migration.  |  Fukui, Y., et al. 2001. Nature. 412: 826-31. PMID: 11518968
2. DOCK2 is essential for antigen-induced translocation of TCR and lipid rafts, but not PKC-theta and LFA-1, in T cells.  |  Sanui, T., et al. 2003. Immunity. 19: 119-29. PMID: 12871644
3. Differential requirements for DOCK2 and phosphoinositide-3-kinase gamma during T and B lymphocyte homing.  |  Nombela-Arrieta, C., et al. 2004. Immunity. 21: 429-41. PMID: 15357953
4. Deletion of DOCK2, a regulator of the actin cytoskeleton in lymphocytes, suppresses cardiac allograft rejection.  |  Jiang, H., et al. 2005. J Exp Med. 202: 1121-30. PMID: 16230477
5. DOCK2 is required in T cell precursors for development of Valpha14 NK T cells.  |  Kunisaki, Y., et al. 2006. J Immunol. 176: 4640-5. PMID: 16585555
6. DOCK2 regulates chemokine-triggered lateral lymphocyte motility but not transendothelial migration.  |  Shulman, Z., et al. 2006. Blood. 108: 2150-8. PMID: 16772603
7. Surflex-Dock 2.1: robust performance from ligand energetic modeling, ring flexibility, and knowledge-based search.  |  Jain, AN. 2007. J Comput Aided Mol Des. 21: 281-306. PMID: 17387436
8. Protein-protein docking with F(2)Dock 2.0 and GB-rerank.  |  Chowdhury, R., et al. 2013. PLoS One. 8: e51307. PMID: 23483883
9. SOS1, ARHGEF1, and DOCK2 rho-GEFs Mediate JAK-Dependent LFA-1 Activation by Chemokines.  |  Toffali, L., et al. 2017. J Immunol. 198: 708-717. PMID: 27986909
10. DOCK2 Promotes Atherosclerosis by Mediating the Endothelial Cell Inflammatory Response.  |  Qian, G., et al. 2024. Am J Pathol. 194: 599-611. PMID: 37838011
11. Identifying MS4A6A+ macrophages as potential contributors to the pathogenesis of nonalcoholic fatty liver disease, periodontitis, and type 2 diabetes mellitus.  |  Wu, J., et al. 2024. Heliyon. 10: e29340. PMID: 38644829
12. Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain.  |  Nagase, T., et al. 1996. DNA Res. 3: 321-9, 341-54. PMID: 9039502

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DOCK 2 Antibody (E-7) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-DOCK 2 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DOCK 2 Antibody (E-7) Specifications

Alternate Product Name: DOCK 2

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 796-894

Epitope Target: DOCK 2

Epitope Species: human

Product Reactivity/Detection: DOCK 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for DOCK 2 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin