DOC4 CRISPR/Cas9 KO Plasmid (h): sc-406806
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DOC4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DOC4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DOC4 CRISPR/Cas9 KO Plasmid (h) | sc-406806 | 20 µg | $397.00 |
Overview
TENM4 encodes teneurin transmembrane protein 4 (DOC4), a large type II membrane protein implicated in cell–cell communication, adhesion, and guidance cues that influence tissue patterning and neuronal connectivity. Through interactions mediated by its extracellular domains and intracellular signaling motifs, DOC4 contributes to cytoskeletal organization, neurite outgrowth, and maintenance of cellular polarity. Altered TENM4 expression or sequence variation has been associated with neurological phenotypes and has also been reported in studies of tumor cell behavior, supporting its relevance to migration and invasive programs. These properties make TENM4 a useful target for dissecting pathways linking adhesion-dependent signaling to development and disease-associated cellular remodeling.
DOC4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TENM4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TENM4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TENM4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DOC4 protein expression.
This CRISPR knockout system enables efficient generation of TENM4-deficient cell models for investigation of DOC4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TENM4 exon(s) critical for DOC4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TENM4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DOC4 CRISPR/Cas9 KO Plasmid (h) and DOC4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TENM4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DOC4 HDR Plasmid (h) and DOC4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TENM4 homology arms to support homology-directed repair at defined TENM4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.