Dnmt3L Antibody (A-4): sc-393603

Dnmt3L Antibody (A-4) is a mouse monoclonal IgG2a antibody that detects Dnmt3L protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Dnmt3L antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dnmt3L protein, which is 387 amino acids in length and contains an ADD-type zinc finger, plays a crucial role in the regulation of gene expression through involvement in DNA methylation processes. Although Dnmt3L lacks intrinsic DNA methyltransferase activity, Dnmt3L remains essential for stimulating de novo methylation by other Dnmt family members, particularly Dnmt3a and Dnmt3b, thereby influencing genomic imprinting and epigenetic regulation. Dnmt3L protein is primarily localized in the nucleus and expressed at low levels in tissues such as the thymus, testis, and ovary. Notably, Dnmt3L interacts with histone deacetylase 1 (HDAC1), which enhances transcriptional repression capabilities, further underscoring significance in epigenetic modifications. Multiple isoforms of Dnmt3L arise from alternative splicing, contributing to the complexity of regulatory functions in cellular processes.

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Dnmt3L Antibody (A-4) References:

1. In vivo activity of murine de novo methyltransferases, Dnmt3a and Dnmt3b.  |  Hsieh, CL. 1999. Mol Cell Biol. 19: 8211-8. PMID: 10567546
2. DNA methyltransferase Dnmt1 associates with histone deacetylase activity.  |  Fuks, F., et al. 2000. Nat Genet. 24: 88-91. PMID: 10615135
3. Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family.  |  Aapola, U., et al. 2000. Genomics. 65: 293-8. PMID: 10857753
4. Stimulation effect of Dnmt3L on the DNA methylation activity of Dnmt3a2.  |  Suetake, I., et al. 2006. J Biochem. 140: 553-9. PMID: 16945937
5. DNMT3L connects unmethylated lysine 4 of histone H3 to de novo methylation of DNA.  |  Ooi, SK., et al. 2007. Nature. 448: 714-7. PMID: 17687327
6. Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation.  |  Jia, D., et al. 2007. Nature. 449: 248-51. PMID: 17713477
7. DNA methylation profile at the DNMT3L promoter: a potential biomarker for cervical cancer.  |  Gokul, G., et al. 2007. Epigenetics. 2: 80-5. PMID: 17965599
8. Structural basis for DNMT3A-mediated de novo DNA methylation.  |  Zhang, ZM., et al. 2018. Nature. 554: 387-391. PMID: 29414941
9. DNMT3L inhibits hepatocellular carcinoma progression through DNA methylation of CDO1: insights from big data to basic research.  |  Yan, X., et al. 2024. J Transl Med. 22: 128. PMID: 38308276
10. Brainwide silencing of prion protein by AAV-mediated delivery of an engineered compact epigenetic editor.  |  Neumann, EN., et al. 2024. Science. 384: ado7082. PMID: 38935715
11. DNA (cytosine-5)-methyltransferases in mouse cells and tissues. Studies with a mechanism-based probe.  |  Yoder, JA., et al. 1997. J Mol Biol. 270: 385-95. PMID: 9237905
12. Cytosine methylation and mammalian development.  |  Walsh, CP. and Bestor, TH. 1999. Genes Dev. 13: 26-34. PMID: 9887097

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dnmt3L Antibody (A-4) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Dnmt3L Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dnmt3L Antibody (A-4) Specifications

Alternate Product Name: DNMT

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 303-387

Epitope Target: Dnmt3L

Epitope Species: human

Product Reactivity/Detection: Dnmt3L

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Dnmt3L Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin