Dnmt1 Antibody (H-12): sc-271729
- Dnmt1 Antibody (H-12) is a mouse monoclonal IgG1 κ Dnmt1 antibody, cited in 72 publications, provided at 200 µg/ml
- raised against amino acids 1317-1616 mapping near the C-terminus of Dnmt1 of human origin
- Dnmt1 Antibody (H-12) is recommended for detection of Dnmt1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Dnmt1 Antibody (H-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Dnmt1 Antibody (H-12) for WB and IHC(P) applications. These reagents are now offered in bundles with Dnmt1 Antibody (H-12) (see ordering information below).
QUICK LINKS
Dnmt1 Antibody (H-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dnmt1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Dnmt1 antibody (H-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dnmt1 plays a crucial role in maintaining DNA methylation patterns, which are essential for regulating gene expression and cellular differentiation. Specifically, Dnmt1 (H-12) monoclonal antibody recognizes the protein responsible for copying methylation marks during DNA replication, ensuring epigenetic information is faithfully inherited by daughter cells. This function is particularly important during development and in stem cells, where precise gene regulation is necessary for proper differentiation and function. Dysregulation of Dnmt1 has been implicated in various diseases, including cancer, where abnormal methylation patterns can lead to the silencing of tumor suppressor genes. Understanding Dnmt1′s role in these processes highlights its significance as a target for therapeutic interventions aimed at restoring normal gene expression patterns.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Dnmt1 Antibody (H-12) References:
- DNA methyltransferase is actively retained in the cytoplasm during early development. | Cardoso, MC. and Leonhardt, H. 1999. J Cell Biol. 147: 25-32. PMID: 10508852
- In vivo activity of murine de novo methyltransferases, Dnmt3a and Dnmt3b. | Hsieh, CL. 1999. Mol Cell Biol. 19: 8211-8. PMID: 10567546
- DNA methyltransferase Dnmt1 associates with histone deacetylase activity. | Fuks, F., et al. 2000. Nat Genet. 24: 88-91. PMID: 10615135
- Transcriptional regulation of the human DNA Methyltransferase (dnmt1) gene. | Bigey, P., et al. 2000. Gene. 242: 407-18. PMID: 10721735
- Mutation-induced DNMT1 cleavage drives neurodegenerative disease. | Wang, W., et al. 2021. Sci Adv. 7: eabe8511. PMID: 34516921
- DNMT1 facilitates growth of breast cancer by inducing MEG3 hyper-methylation. | Zhu, X., et al. 2022. Cancer Cell Int. 22: 56. PMID: 35109842
- DNMT1/miR-130a/ZEB1 Regulatory Pathway Affects the Inflammatory Response in Lipopolysaccharide-Induced Sepsis. | Ding, J., et al. 2022. DNA Cell Biol. 41: 479-486. PMID: 35486848
- GSK-3484862 targets DNMT1 for degradation in cells. | Chen, Q., et al. 2023. NAR Cancer. 5: zcad022. PMID: 37206360
- Insights into DNMT1 and programmed cell death in diseases. | Yan, L., et al. 2023. Biomed Pharmacother. 168: 115753. PMID: 37871559
- DNA (cytosine-5)-methyltransferases in mouse cells and tissues. Studies with a mechanism-based probe. | Yoder, JA., et al. 1997. J Mol Biol. 270: 385-95. PMID: 9237905
- Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells. | Okano, M., et al. 1998. Nucleic Acids Res. 26: 2536-40. PMID: 9592134
- Cytosine methylation and mammalian development. | Walsh, CP. and Bestor, TH. 1999. Genes Dev. 13: 26-34. PMID: 9887097
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dnmt1 Antibody (H-12) | sc-271729 | 200 µg/ml | $316.00 | |||
Dnmt1 Antibody (H-12): m-IgG Fc BP-HRP Bundle | sc-527283 | 200 µg Ab; 10 µg BP | $354.00 | |||
Dnmt1 Antibody (H-12): m-IgG1 BP-HRP Bundle | sc-532656 | 200 µg Ab; 20 µg BP | $354.00 | |||
Dnmt1 Antibody (H-12) AC | sc-271729 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Dnmt1 Antibody (H-12) HRP | sc-271729 HRP | 200 µg/ml | $316.00 | |||
Dnmt1 Antibody (H-12) FITC | sc-271729 FITC | 200 µg/ml | $330.00 | |||
Dnmt1 Antibody (H-12) PE | sc-271729 PE | 200 µg/ml | $343.00 | |||
Dnmt1 Antibody (H-12) Alexa Fluor® 488 | sc-271729 AF488 | 200 µg/ml | $357.00 | |||
Dnmt1 Antibody (H-12) Alexa Fluor® 546 | sc-271729 AF546 | 200 µg/ml | $357.00 | |||
Dnmt1 Antibody (H-12) Alexa Fluor® 594 | sc-271729 AF594 | 200 µg/ml | $357.00 | |||
Dnmt1 Antibody (H-12) Alexa Fluor® 647 | sc-271729 AF647 | 200 µg/ml | $357.00 | |||
Dnmt1 Antibody (H-12) Alexa Fluor® 680 | sc-271729 AF680 | 200 µg/ml | $357.00 | |||
Dnmt1 Antibody (H-12) Alexa Fluor® 790 | sc-271729 AF790 | 200 µg/ml | $357.00 |