DND1 Double Nickase Plasmid (h): sc-404161-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DND1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DND1 Double Nickase Plasmid (h) and DND1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DND1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DND1 Antibody (X22): sc-130493
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DND1 Double Nickase Plasmid (h) | sc-404161-NIC | 20 µg | $410.00 | |||
DND1 Double Nickase Plasmid (h2) | sc-404161-NIC-2 | 20 µg | $410.00 |
DND1 (dead end homolog 1) encodes an RNA-binding protein that modulates post-transcriptional gene regulation by binding specific mRNAs and influencing their stability and translation. It is best characterized in germ cell biology, where it contributes to maintenance of germ cell identity and supports proper differentiation by buffering gene expression programs. DND1 can counter microRNA-mediated repression on selected transcripts, linking it to broader networks controlling RNA turnover and developmental timing. Dysregulation of DND1-associated RNA regulatory circuits has been connected to aberrant germ cell development and has been studied in the context of germ cell tumor susceptibility and related oncogenic programs.
DND1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DND1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DND1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DND1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DND1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.