DnaJC12 CRISPR/Cas9 KO Plasmid (h): sc-412707
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DnaJC12 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DnaJC12 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DnaJC12 CRISPR/Cas9 KO Plasmid (h) | sc-412707 | 20 µg | $397.00 |
Overview
DNAJC12 encodes DnaJC12, a J-domain co-chaperone that partners with HSP70 machinery to regulate protein folding and proteostasis within the cytosol and endoplasmic reticulum. By stimulating HSP70 ATPase activity, DnaJC12 supports maturation and stability of client proteins and helps coordinate cellular responses to proteotoxic stress. This chaperone network interfaces with ubiquitin–proteasome and ER-associated degradation pathways to maintain protein quality control. Altered DNAJC12 function has been linked to disrupted enzyme homeostasis and neuro-metabolic phenotypes, making it relevant for studying stress adaptation and protein maturation pathways in human cells.
DnaJC12 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DNAJC12 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DNAJC12 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DNAJC12 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DnaJC12 protein expression.
This CRISPR knockout system enables efficient generation of DNAJC12-deficient cell models for investigation of DnaJC12 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DNAJC12 exon(s) critical for DnaJC12 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DNAJC12 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DnaJC12 CRISPR/Cas9 KO Plasmid (h) and DnaJC12 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DNAJC12 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DnaJC12 HDR Plasmid (h) and DnaJC12 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DNAJC12 homology arms to support homology-directed repair at defined DNAJC12 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.