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DnaJC1 Antibody (D-10): sc-514244

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Datasheets
  • DnaJC1 Antibody (D-10) is a mouse monoclonal IgG1 κ DnaJC1 antibody, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 255-554 mapping at the C-terminus of DnaJC1 of human origin
  • DnaJC1 Antibody (D-10) is recommended for detection of DnaJC1 of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-DnaJC1 Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • TransCruz reagent for ChIP application (sc-514244 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for DnaJC1 Antibody (D-10) for WB applications. These reagents are now offered in bundles with DnaJC1 Antibody (D-10) (see ordering information below).

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SEE ALSO...

DnaJC1 Antibody (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects DnaJC1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-DnaJC1 antibody (D-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DnaJC1, also known as DnaJ homolog subfamily C member 1, plays a vital role in cellular protein homeostasis by functioning as a molecular chaperone. DnaJC1 is primarily located in the endoplasmic reticulum, nucleus, and microsome, where DnaJC1 assists in the proper folding of nascent polypeptides and the refolding of misfolded proteins. DnaJC1′s presence in these cellular compartments is crucial, as DnaJC1 helps maintain protein quality control, which is essential for cellular function and survival. DnaJC1 contains a J domain that interacts with HSP 70, facilitating ATP hydrolysis, and two SANT domains that bind to GRP 78 and AACT, respectively. Additionally, through a cytosolic domain, DnaJC1 interacts with ribosomes, suggesting a role in modulating protein synthesis. DnaJC1 gene is located on human chromosome 10p12.31 and mouse chromosome 2 A3, highlighting evolutionary conservation and importance across species.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

DnaJC1 Antibody (D-10) References:

  1. Interaction of murine BiP/GRP78 with the DnaJ homologue MTJ1.  |  Chevalier, M., et al. 2000. J Biol Chem. 275: 19620-7. PMID: 10777498
  2. Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region.  |  Lehner, B., et al. 2004. Genomics. 83: 153-67. PMID: 14667819
  3. The SANT2 domain of the murine tumor cell DnaJ-like protein 1 human homologue interacts with alpha1-antichymotrypsin and kinetically interferes with its serpin inhibitory activity.  |  Kroczynska, B., et al. 2004. J Biol Chem. 279: 11432-43. PMID: 14668352
  4. BIP co-chaperone MTJ1/ERDJ1 interacts with inter-alpha-trypsin inhibitor heavy chain 4.  |  Kroczynska, B., et al. 2005. Biochem Biophys Res Commun. 338: 1467-77. PMID: 16271702

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

DnaJC1 Antibody (D-10)

sc-514244
200 µg/ml
$316.00

DnaJC1 Antibody (D-10): m-IgG Fc BP-HRP Bundle

sc-527874
200 µg Ab; 10 µg BP
$354.00

DnaJC1 Antibody (D-10): m-IgG1 BP-HRP Bundle

sc-533247
200 µg Ab; 20 µg BP
$354.00

DnaJC1 Antibody (D-10) X

sc-514244 X
200 µg/0.1 ml
$316.00

DnaJC1 Antibody (D-10) AC

sc-514244 AC
500 µg/ml, 25% agarose
$416.00

DnaJC1 Antibody (D-10) HRP

sc-514244 HRP
200 µg/ml
$316.00

DnaJC1 Antibody (D-10) FITC

sc-514244 FITC
200 µg/ml
$330.00

DnaJC1 Antibody (D-10) PE

sc-514244 PE
200 µg/ml
$343.00

DnaJC1 Antibody (D-10) Alexa Fluor® 488

sc-514244 AF488
200 µg/ml
$357.00

DnaJC1 Antibody (D-10) Alexa Fluor® 546

sc-514244 AF546
200 µg/ml
$357.00

DnaJC1 Antibody (D-10) Alexa Fluor® 594

sc-514244 AF594
200 µg/ml
$357.00

DnaJC1 Antibody (D-10) Alexa Fluor® 647

sc-514244 AF647
200 µg/ml
$357.00

DnaJC1 Antibody (D-10) Alexa Fluor® 680

sc-514244 AF680
200 µg/ml
$357.00

DnaJC1 Antibody (D-10) Alexa Fluor® 790

sc-514244 AF790
200 µg/ml
$357.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with DnaJC1 (D-10): sc-514244 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_10, d_17, h_7CST
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Rated 5 out of 5 by from Produced positive Western blot data of DnaJC1Produced positive Western blot data of DnaJC1 expression in non-transfected 293T, human DnaJC1 transfected 293T K-562, HeLa and RT-4 whole cell lysates. -SCBT QC
Date published: 2014-12-17
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DnaJC1 Antibody (D-10) is rated 5.0 out of 5 by 1.
  • y_2025, m_10, d_17, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_514244, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 118ms
  • REVIEWS, PRODUCT