Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DnaJB6 CRISPR/Cas9 KO Plasmid (h): sc-404227
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DnaJB6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DnaJB6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DnaJB6 Antibody (B-5): sc-365574
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DnaJB6 CRISPR/Cas9 KO Plasmid (h) | sc-404227 | 20 µg | $397.00 |
Overview
DNAJB6 encodes DnaJB6, a member of the Hsp40/DnaJ co-chaperone family that cooperates with Hsp70 to regulate protein folding, triage, and proteostasis under basal and stress conditions. DnaJB6 is implicated in suppressing aggregation of misfolded proteins and contributes to cytoskeletal organization, chaperone-assisted quality control, and stress-responsive signaling that influence cellular homeostasis. Perturbation of DNAJB6 function has been linked to disorders characterized by protein aggregation and muscle pathology, including limb-girdle muscular dystrophy, and is also studied in the context of neurodegeneration-relevant proteotoxic stress. These biology connections make DNAJB6 a useful node for dissecting pathways that govern aggregation, chaperone networks, and cellular resilience to misfolded proteins.
DnaJB6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DNAJB6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DNAJB6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DNAJB6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DnaJB6 protein expression.
This CRISPR knockout system enables efficient generation of DNAJB6-deficient cell models for investigation of DnaJB6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DNAJB6 exon(s) critical for DnaJB6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DNAJB6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DnaJB6 CRISPR/Cas9 KO Plasmid (h) and DnaJB6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DNAJB6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DnaJB6 HDR Plasmid (h) and DnaJB6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DNAJB6 homology arms to support homology-directed repair at defined DNAJB6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.