DNA pol ι Antibody (NN7): sc-101026

DNA pol ι Antibody (NN7) is a mouse monoclonal IgG3 kappa light chain antibody that detects DNA pol ι of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-DNA pol ι antibody (NN7) is available as a non-conjugated format. DNA pol ι, encoded by the POLI gene located on human chromosome 18q21.2, plays a critical role in DNA replication and repair processes, particularly with damaged DNA. DNA pol ι (NN7) monoclonal antibody recognizes a protein known for bypassing DNA lesions that would otherwise stall replication, a function crucial for maintaining genomic stability. DNA pol ι demonstrates a high error rate when replicating undamaged DNA, often misincorporating guanine instead of adenosine at thymine templates. Such error-prone characteristics can lead to mutations, making DNA pol ι a key factor in cancer-associated mutagenesis. DNA pol ι works alongside DNA polymerase ζ to facilitate DNA lesion bypass, highlighting a crucial role in cellular DNA damage response.

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DNA pol ι Antibody (NN7) References:

1. poliota, a remarkably error-prone human DNA polymerase.  |  Tissier, A., et al. 2000. Genes Dev. 14: 1642-50. PMID: 10887158
2. Preferential incorporation of G opposite template T by the low-fidelity human DNA polymerase iota.  |  Zhang, Y., et al. 2000. Mol Cell Biol. 20: 7099-108. PMID: 10982826
3. Eukaryotic polymerases iota and zeta act sequentially to bypass DNA lesions.  |  Johnson, RE., et al. 2000. Nature. 406: 1015-9. PMID: 10984059
4. Misinsertion and bypass of thymine-thymine dimers by human DNA polymerase iota.  |  Tissier, A., et al. 2000. EMBO J. 19: 5259-66. PMID: 11013228
5. Novel human and mouse homologs of Saccharomyces cerevisiae DNA polymerase eta.  |  McDonald, JP., et al. 1999. Genomics. 60: 20-30. PMID: 10458907

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DNA pol ι Antibody (NN7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-DNA pol ι Antibody (NN7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DNA pol ι Antibody (NN7) Specifications

Alternate Product Name: DNA pol ι

Product Clone Name: NN7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 616-716

Epitope Target: DNA pol ι

Epitope Species: human

Product Reactivity/Detection: DNA pol ι

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 50 µg IgG₃ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for DNA pol ι Antibody (NN7): N/A