Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DNA pol β Double Nickase Plasmid (h): sc-402861-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DNA pol β Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DNA pol β Double Nickase Plasmid (h) and DNA pol β Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting POLB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DNA pol β Antibody (D-11): sc-376581
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DNA pol β Double Nickase Plasmid (h) | sc-402861-NIC | 20 µg | $410.00 | |||
DNA pol β Double Nickase Plasmid (h2) | sc-402861-NIC-2 | 20 µg | $410.00 |
POLB encodes DNA polymerase beta (DNA pol β), a key enzyme in the base excision repair (BER) pathway that fills single-nucleotide gaps and processes 5′-deoxyribose phosphate intermediates during repair synthesis. Through coordinated action with XRCC1, DNA ligase III, and DNA glycosylases, DNA pol β helps maintain genome integrity after oxidative damage, alkylation, and spontaneous base loss. Altered POLB activity or expression has been linked to increased mutagenesis and chromosomal instability, connecting this pathway to mechanisms that contribute to cancer biology and age-associated genomic decline. As a central BER factor, DNA pol β is frequently studied for its impact on DNA damage signaling, replication stress responses, and cellular sensitivity to genotoxic stressors.
DNA pol β Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the POLB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within POLB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt POLB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of POLB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.