DNA-PK_CS Antibody (G-4): sc-5282

DNA-PKCS Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects DNA-PKCS protein of human origin through applications such as western blotting, immunoprecipitation, immunofluorescence, and enzyme-linked immunosorbent assay. DNA-PKCS (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. DNA-PKCS, also known as DNA-dependent protein kinase catalytic subunit, plays a crucial role in the repair of DNA double-strand breaks (DSBs) and is essential for V(D)J recombination, a process vital for the development of immune diversity. Located in the nucleus, DNA-PKCS forms a heterotrimeric complex with Ku proteins, which are responsible for recognizing and binding to DSBs, thereby facilitating repair processes. Proper functioning of DNA-PKCS is critical, as defects can lead to severe consequences, including increased sensitivity to ionizing radiation and impaired DNA repair mechanisms, which are associated with various genetic disorders and cancers. DNA-PKCS monoclonal antibody (G-4) enables researchers to gain valuable insights into mechanisms of DNA repair and implications of DNA-PKCS in cellular responses to DNA damage.

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DNA-PK_CS Antibody (G-4) References:

1. Induction of apoptosis and autophagic cell death by the vanillin derivative 6-bromine-5-hydroxy-4-methoxybenzaldehyde is accompanied by the cleavage of DNA-PKcs and rapid destruction of c-Myc oncoprotein in HepG2 cells.  |  Yan, YQ., et al. 2007. Cancer Lett. 252: 280-9. PMID: 17316978
2. The mutagenic potential of non-homologous end joining in the absence of the NHEJ core factors Ku70/80, DNA-PKcs and XRCC4-LigIV.  |  Kuhfittig-Kulle, S., et al. 2007. Mutagenesis. 22: 217-33. PMID: 17347130
3. [Expression of PTEN mRNA, hTERT and DNA-PKcs in bone marrow cells of children with acute leukemia].  |  Chai, YH., et al. 2007. Zhonghua Er Ke Za Zhi. 45: 74-5. PMID: 17349160
4. Role of DNA-PKcs in the bystander effect after low- or high-LET irradiation.  |  Kanasugi, Y., et al. 2007. Int J Radiat Biol. 83: 73-80. PMID: 17357428
5. DNA-PKcs promotes fork reversal and chemoresistance.  |  Dibitetto, D., et al. 2022. Mol Cell. 82: 3932-3942.e6. PMID: 36130596
6. DNA-PKcs promotes sepsis-induced multiple organ failure by triggering mitochondrial dysfunction.  |  Zou, R., et al. 2022. J Adv Res. 41: 39-48. PMID: 36328752
7. TBC1D15 deficiency protects against doxorubicin cardiotoxicity via inhibiting DNA-PKcs cytosolic retention and DNA damage.  |  Yu, W., et al. 2023. Acta Pharm Sin B. 13: 4823-4839. PMID: 38045047
8. When is a lipid kinase not a lipid kinase? When it is a protein kinase.  |  Hunter, T. 1995. Cell. 83: 1-4. PMID: 7553860
9. PIK-related kinases: DNA repair, recombination, and cell cycle checkpoints.  |  Keith, CT. and Schreiber, SL. 1995. Science. 270: 50-1. PMID: 7569949
10. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product.  |  Hartley, KO., et al. 1995. Cell. 82: 849-56. PMID: 7671312
11. Discovery of AT gene sparks biomedical research bonanza.  |  Nowak, R. 1995. Science. 268: 1700-1. PMID: 7792589
12. A single ataxia telangiectasia gene with a product similar to PI-3 kinase.  |  Savitsky, K., et al. 1995. Science. 268: 1749-53. PMID: 7792600

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DNA-PK_CS Antibody (G-4) has 79 citations in a variety of scientific publications.

Primary antibodies like Anti-DNA-PK_CS Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DNA-PK_CS Antibody (G-4) Specifications

Alternate Product Name: DNA-PKCS

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2965-4127

Epitope Target: DNA-PKCS

Epitope Species: human

Product Reactivity/Detection: DNA-PKcs

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 79 citations in various scientific publications

Available Conjugates/Forms for DNA-PK_CS Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin