Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dlx-1 Double Nickase Plasmid (h): sc-405307-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dlx-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dlx-1 Double Nickase Plasmid (h) and Dlx-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DLX1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dlx-1 Antibody (F-16): sc-81959
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dlx-1 Double Nickase Plasmid (h) | sc-405307-NIC | 20 µg | $410.00 | |||
Dlx-1 Double Nickase Plasmid (h2) | sc-405307-NIC-2 | 20 µg | $410.00 |
DLX1 encodes the homeobox transcription factor Dlx-1, a nuclear regulator essential for embryonic patterning and lineage specification, particularly in craniofacial structures and forebrain interneuron development. Dlx-1 modulates gene programs controlling differentiation, migration, and maturation of neural and ectoderm-derived progenitors through sequence-specific DNA binding and cooperation with other developmental transcription factors. In adult and disease contexts, altered DLX1 expression has been associated with dysregulated cell state transitions and aberrant transcriptional networks reported in cancers and neurodevelopmental disorders, supporting its use as a mechanistic node in gene regulatory studies. DLX1 is therefore widely studied for its contribution to developmental gene expression cascades and context-dependent remodeling of cellular identity.
Dlx-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DLX1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DLX1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DLX1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DLX1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.