DLD Antibody (G-2): sc-365977

DLD Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects DLD protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DLD Antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DLD, or Dihydrolipoyl dehydrogenase, is a crucial enzyme in cellular metabolism, functioning as a flavin-dependent oxidoreductase that plays a vital role in the mitochondrial matrix. DLD (G-2) antibody recognizes a key component of several multi-enzyme complexes, including the pyruvate dehydrogenase complex and the branched-chain α-keto acid dehydrogenase complex, which are essential for the oxidative decarboxylation of α-keto acids. DLD exists in various oligomeric forms, such as monomers, homodimers, and tetramers, which are important for enzymatic activity and stability. DLD catalyzes the conversion of dihydrolipoic acid and NAD+ into NADH and lipoic acid, thereby contributing to the generation of reducing equivalents necessary for ATP production. Mutations in the DLD gene can lead to severe metabolic disorders, including maple syrup urine disease and congenital infantile lactic acidosis, highlighting DLD′s critical role in energy metabolism and overall cellular function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DLD Antibody (G-2) References:

1. Mitochondrial alpha-ketoglutarate dehydrogenase complex generates reactive oxygen species.  |  Starkov, AA., et al. 2004. J Neurosci. 24: 7779-88. PMID: 15356189
2. Association of the dihydrolipoamide dehydrogenase gene with Alzheimer's disease in an Ashkenazi Jewish population.  |  Brown, AM., et al. 2004. Am J Med Genet B Neuropsychiatr Genet. 131B: 60-6. PMID: 15389771
3. How dihydrolipoamide dehydrogenase-binding protein binds dihydrolipoamide dehydrogenase in the human pyruvate dehydrogenase complex.  |  Ciszak, EM., et al. 2006. J Biol Chem. 281: 648-55. PMID: 16263718
4. Activity of human dihydrolipoamide dehydrogenase is largely reduced by mutation at isoleucine-51 to alanine.  |  Kim, H. 2006. J Biochem Mol Biol. 39: 223-7. PMID: 16584639
5. A new level of architectural complexity in the human pyruvate dehydrogenase complex.  |  Smolle, M., et al. 2006. J Biol Chem. 281: 19772-80. PMID: 16679318
6. Novel mutations in dihydrolipoamide dehydrogenase deficiency in two cousins with borderline-normal PDH complex activity.  |  Cameron, JM., et al. 2006. Am J Med Genet A. 140: 1542-52. PMID: 16770810
7. Thermal unfolding process of dihydrolipoamide dehydrogenase studied by fluorescence spectroscopy.  |  Nishimoto, E., et al. 2006. J Biochem. 140: 349-57. PMID: 16861247
8. Testing for linkage and association across the dihydrolipoyl dehydrogenase gene region with Alzheimer's disease in three sample populations.  |  Brown, AM., et al. 2007. Neurochem Res. 32: 857-69. PMID: 17342416
9. Cryptic proteolytic activity of dihydrolipoamide dehydrogenase.  |  Babady, NE., et al. 2007. Proc Natl Acad Sci U S A. 104: 6158-63. PMID: 17404228

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DLD Antibody (G-2) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-DLD Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DLD Antibody (G-2) Specifications

Alternate Product Name: DLD

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 356-509

Epitope Target: DLD

Epitope Species: human

Product Reactivity/Detection: DLD

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for DLD Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin