Dist1 CRISPR/Cas9 KO Plasmid (h): sc-406805
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dist1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Dist1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dist1 CRISPR/Cas9 KO Plasmid (h) | sc-406805 | 20 µg | $397.00 |
Overview
RHBDF1 (Dist1) encodes an inactive rhomboid family member that functions as a regulatory cofactor for ER-resident quality control and growth factor signaling. Dist1 is implicated in control of ADAM17-dependent ectodomain shedding, influencing EGFR ligand release and downstream MAPK/ERK and PI3K/AKT pathway activity, as well as cellular stress responses and inflammatory signaling. Through these processes, RHBDF1 contributes to epithelial homeostasis, cell survival, and migration programs. Dysregulated RHBDF1 expression or activity has been associated with altered proliferative signaling and disease-relevant phenotypes in cancer and inflammatory contexts, supporting its utility as a mechanistic target in pathway studies.
Dist1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RHBDF1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RHBDF1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RHBDF1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dist1 protein expression.
This CRISPR knockout system enables efficient generation of RHBDF1-deficient cell models for investigation of Dist1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RHBDF1 exon(s) critical for Dist1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RHBDF1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Dist1 CRISPR/Cas9 KO Plasmid (h) and Dist1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RHBDF1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Dist1 HDR Plasmid (h) and Dist1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RHBDF1 homology arms to support homology-directed repair at defined RHBDF1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.