DISP2 CRISPR/Cas9 KO Plasmid (m): sc-431923
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DISP2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DISP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DISP2 CRISPR/Cas9 KO Plasmid (m) | sc-431923 | 20 µg | $397.00 |
Overview
Dispatched RND transporter family member 2 (DISP2), encoded by the mouse Disp2 gene, is a multipass membrane protein related to the Dispatched family implicated in the release and extracellular distribution of lipid-modified Hedgehog ligands. Through modulation of Hedgehog morphogen availability, DISP2 is positioned to influence developmental patterning programs, epithelial–mesenchymal signaling, and tissue homeostasis governed by Hedgehog pathway activity. Altered Hedgehog signaling dynamics are relevant to studies of congenital developmental abnormalities and cancer-associated signaling reprogramming, making Disp2 a useful entry point for dissecting pathway regulation. In mouse models, Disp2 perturbation can be leveraged to investigate how membrane transport processes shape paracrine signaling gradients and downstream transcriptional outputs.
DISP2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Disp2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Disp2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Disp2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DISP2 protein expression.
This CRISPR knockout system enables efficient generation of Disp2-deficient cell models for investigation of DISP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Disp2 exon(s) critical for DISP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Disp2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DISP2 CRISPR/Cas9 KO Plasmid (m) and DISP2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Disp2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DISP2 HDR Plasmid (m) and DISP2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Disp2 homology arms to support homology-directed repair at defined Disp2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.