DIO1 Double Nickase Plasmid (h): sc-405162-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DIO1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DIO1 Double Nickase Plasmid (h) and DIO1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DIO1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DIO1 Antibody (B-7): sc-515198
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DIO1 Double Nickase Plasmid (h) | sc-405162-NIC | 20 µg | $410.00 | |||
DIO1 Double Nickase Plasmid (h2) | sc-405162-NIC-2 | 20 µg | $410.00 |
DIO1 encodes type I iodothyronine deiodinase, a selenoenzyme that catalyzes outer- and inner-ring deiodination of thyroid hormones, converting thyroxine (T4) to the bioactive triiodothyronine (T3) and regulating local thyroid hormone availability. This activity supports endocrine homeostasis and shapes transcriptional programs controlled by thyroid hormone receptors, linking DIO1 function to metabolic rate, lipid and carbohydrate metabolism, and redox-sensitive cellular processes. DIO1 expression is prominent in liver and kidney and is modulated by nutritional status and hormonal cues that influence thyroid hormone signaling dynamics. Dysregulated deiodinase activity is associated with altered thyroid hormone profiles observed in thyroid disorders and systemic metabolic perturbations, making DIO1 a relevant target for mechanistic studies of hormone-dependent pathways.
DIO1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DIO1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DIO1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DIO1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DIO1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.