Dio-1 Antibody (B-9): sc-25264

Dio-1 Antibody (B-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects Dio-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Dio-1 (B-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dio-1, also known as Death Inducer-Obliterator-1, is a putative transcription factor characterized by two zinc finger motifs, which are crucial for DNA-binding capability. Dio-1 plays a significant role in regulating apoptosis, particularly during limb development, where Dio-1 translocates to the nucleus to activate programmed cell death. The precise regulation of apoptosis is vital for shaping and defining digits, as apoptosis determines tissue presence. Dio-1 expression increases in response to apoptotic signals, leading to apoptosis induction, a process similarly influenced by NFκB and nerve growth factor expression in reaction to external stimuli. Additionally, caspase inhibitors and Bcl-2 expression modulate Dio-1 activity, reinforcing Dio-1′s critical function in initiating programmed cell death.

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Dio-1 Antibody (B-9) References:

1. DIO-1 is a gene involved in onset of apoptosis in vitro, whose misexpression disrupts limb development.  |  García-Domingo, D., et al. 1999. Proc Natl Acad Sci U S A. 96: 7992-7. PMID: 10393935
2. Osteoblast apoptosis and bone turnover.  |  Hock, JM., et al. 2001. J Bone Miner Res. 16: 975-84. PMID: 11393794
3. Death inducer-obliterator 1 triggers apoptosis after nuclear translocation and caspase upregulation.  |  García-Domingo, D., et al. 2003. Mol Cell Biol. 23: 3216-25. PMID: 12697821
4. SPOC: a widely distributed domain associated with cancer, apoptosis and transcription.  |  Sánchez-Pulido, L., et al. 2004. BMC Bioinformatics. 5: 91. PMID: 15239844
5. Gambogic acid induces death inducer-obliterator 1-mediated apoptosis in Jurkat T cells.  |  Wang, Y., et al. 2008. Acta Pharmacol Sin. 29: 349-54. PMID: 18298900
6. Inhibitors of protein synthesis and RNA synthesis prevent neuronal death caused by nerve growth factor deprivation.  |  Martin, DP., et al. 1988. J Cell Biol. 106: 829-44. PMID: 2450099
7. Deiodinases share an evolutionarily conserved thyroid hormone-binding motif.  |  Benvenga, S. and Guarneri, F. 2018. Front Biosci (Landmark Ed). 23: 2195-2203. PMID: 29772555
8. The relationship between inflammatory factors, oxidative stress and DIO-1 concentration in patients with chronic renal failure accompanied with or without euthyroid sick syndrome.  |  Qin, SL., et al. 2018. J Int Med Res. 46: 4061-4070. PMID: 30152254
9. Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy.  |  Zhong, Y., et al. 2023. Nutrients. 15: PMID: 37432297
10. Programmed cell death in animal development.  |  Jacobson, MD., et al. 1997. Cell. 88: 347-54. PMID: 9039261
11. Role of Rel/NF-kappaB transcription factors during the outgrowth of the vertebrate limb.  |  Kanegae, Y., et al. 1998. Nature. 392: 611-4. PMID: 9560158
12. Shaping limbs by apoptosis.  |  Chen, Y. and Zhao, X. 1998. J Exp Zool. 282: 691-702. PMID: 9846381

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dio-1 Antibody (B-9) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Dio-1 Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dio-1 Antibody (B-9) Specifications

Alternate Product Name: Dio-1

Product Clone Name: B-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 315-614

Epitope Target: Dio-1

Epitope Species: mouse

Product Reactivity/Detection: Dio-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Dio-1 Antibody (B-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin