Dim1 Antibody (B-11): sc-514093
- Dim1 Antibody (B-11) is a mouse monoclonal IgG1 κ Dim1 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 119-142 at the C-terminus of Dim1 of human origin
- Dim1 Antibody (B-11) is recommended for detection of Dim1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Dim1 Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Dim1 Antibody (B-11) for WB applications. These reagents are now offered in bundles with Dim1 Antibody (B-11) (see ordering information below).
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Dim1 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dim1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Dim1 antibody (B-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dim1, also known as TXNL4A (Thioredoxin-like protein 4A) or Spliceosomal U5 snRNP-specific 15 kDa protein, is a crucial 142 amino acid protein involved in pre-mRNA splicing, which is essential for proper gene expression. Deletion of Dim1 gene in Schizosaccharomyces pombe results in embryonal lethality during gastrulation due to failure in expressing early zygotic transcripts. Dim1′s sensitivity to the microtubule destabilizing drug thiabendazole suggests a role in mitotic spindle formation, highlighting significance in G2/M progression of the cell cycle and chromosomal segregation during mitosis. Localized primarily in the nucleus, Dim1 interacts with several proteins, including heterogeneous nuclear ribonucleoprotein F, heterogeneous nuclear ribonucleoprotein H2, Cas-L, and PQBP-1, to regulate gene expression effectively. This multifaceted role in cellular processes makes Dim1 (B-11) antibody an invaluable tool for researchers studying gene regulation and cell cycle dynamics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Dim1 Antibody (B-11) References:
- Identification, characterization and crystal structure analysis of the human spliceosomal U5 snRNP-specific 15 kD protein. | Reuter, K., et al. 1999. J Mol Biol. 294: 515-25. PMID: 10610776
- The evolutionarily conserved Dim1 protein defines a novel branch of the thioredoxin fold superfamily. | Zhang, YZ., et al. 1999. Physiol Genomics. 1: 109-18. PMID: 11015569
- Evidence that dim1 associates with proteins involved in pre-mRNA splicing, and delineation of residues essential for dim1 interactions with hnRNP F and Npw38/PQBP-1. | Zhang, Y., et al. 2000. Gene. 257: 33-43. PMID: 11054566
- Structure, stability, and function of hDim1 investigated by NMR, circular dichroism, and mutational analysis. | Zhang, YZ., et al. 2003. Biochemistry. 42: 9609-18. PMID: 12911302
- Dim1p is required for efficient splicing and export of mRNA encoding lid1p, a component of the fission yeast anaphase-promoting complex. | Carnahan, RH., et al. 2005. Eukaryot Cell. 4: 577-87. PMID: 15755920
- The Dim protein family: from structure to splicing. | Simeoni, F. and Divita, G. 2007. Cell Mol Life Sci. 64: 2079-89. PMID: 17558560
- Fission yeast dim1(+) encodes a functionally conserved polypeptide essential for mitosis. | Berry, LD. and Gould, KL. 1997. J Cell Biol. 137: 1337-54. PMID: 9182666
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dim1 Antibody (B-11) | sc-514093 | 200 µg/ml | $322.00 | |||
Dim1 Antibody (B-11): m-IgG Fc BP-HRP Bundle | sc-527851 | 200 µg Ab; 10 µg BP | $361.00 | |||
Dim1 Antibody (B-11): m-IgG1 BP-HRP Bundle | sc-533224 | 200 µg Ab; 20 µg BP | $361.00 | |||
Dim1 Antibody (B-11) AC | sc-514093 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Dim1 Antibody (B-11) HRP | sc-514093 HRP | 200 µg/ml | $322.00 | |||
Dim1 Antibody (B-11) FITC | sc-514093 FITC | 200 µg/ml | $336.00 | |||
Dim1 Antibody (B-11) PE | sc-514093 PE | 200 µg/ml | $349.00 | |||
Dim1 Antibody (B-11) Alexa Fluor® 488 | sc-514093 AF488 | 200 µg/ml | $364.00 | |||
Dim1 Antibody (B-11) Alexa Fluor® 546 | sc-514093 AF546 | 200 µg/ml | $364.00 | |||
Dim1 Antibody (B-11) Alexa Fluor® 594 | sc-514093 AF594 | 200 µg/ml | $364.00 | |||
Dim1 Antibody (B-11) Alexa Fluor® 647 | sc-514093 AF647 | 200 µg/ml | $364.00 | |||
Dim1 Antibody (B-11) Alexa Fluor® 680 | sc-514093 AF680 | 200 µg/ml | $364.00 | |||
Dim1 Antibody (B-11) Alexa Fluor® 790 | sc-514093 AF790 | 200 µg/ml | $364.00 | |||
Dim1 (B-11) Neutralizing Peptide | sc-514093 P | 100 µg/0.5 ml | $69.00 |