DIEXF CRISPR/Cas9 KO Plasmid (h): sc-411950
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DIEXF CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DIEXF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DIEXF CRISPR/Cas9 KO Plasmid (h) | sc-411950 | 20 µg | $397.00 |
Overview
DIEXF encodes a conserved RNA-associated protein implicated in ribosome biogenesis and nucleolar function, supporting the processing and maturation of precursor rRNA required for efficient protein synthesis. By influencing ribosomal assembly and translational capacity, DIEXF contributes to fundamental controls of cell growth, cell-cycle progression, and stress-adaptive gene expression programs. Perturbation of ribosome biogenesis factors is frequently linked to genomic instability and altered proteostasis, processes that shape cellular phenotypes in developmental disorders and cancer-related contexts. Accordingly, DIEXF is studied as part of core RNA metabolism pathways that coordinate nucleolar homeostasis with proliferation and differentiation.
DIEXF CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DIEXF gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DIEXF together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DIEXF open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DIEXF protein expression.
This CRISPR knockout system enables efficient generation of DIEXF-deficient cell models for investigation of DIEXF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DIEXF exon(s) critical for DIEXF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DIEXF genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DIEXF CRISPR/Cas9 KO Plasmid (h) and DIEXF CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DIEXF locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DIEXF HDR Plasmid (h) and DIEXF HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DIEXF homology arms to support homology-directed repair at defined DIEXF target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.