DHS Antibody (G-12): sc-365076
- DHS Antibody (G-12) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 70-369 mapping at the C-terminus of DHS of human origin
- recommended for detection of DHS of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DHS Antibody (G-12) for WB applications. These reagents are now offered in bundles with DHS Antibody (G-12) (see ordering information below).
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DHS Antibody (G-12) is a mouse monoclonal IgG2a antibody that detects deoxyhypusine synthase (DHS) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DHS plays a pivotal role in the post-translational modification of eukaryotic initiation factor 5A (eIF-5A) by catalyzing the formation of hypusine, a unique modification that is critical for eIF-5A′s function in promoting cell proliferation and regulating various cellular processes. The importance of hypusine lies in eIF-5A′s ability to facilitate the translation of specific mRNAs, which are essential for cell growth and differentiation. By understanding DHS function and hypusine biosynthesis, researchers can gain insights into cell cycle regulation mechanisms and potential implications in diseases such as cancer, where eIF-5A activity is often dysregulated. Anti-DHS antibody (G-12) serves as an invaluable tool for studying these processes and advancing cellular biology knowledge.
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DHS Antibody (G-12) References:
- Deoxyhypusine synthase is phosphorylated by protein kinase C in vivo as well as in vitro. | Kang, KR., et al. 2002. Exp Mol Med. 34: 489-95. PMID: 12526092
- Reversal of the deoxyhypusine synthesis reaction. Generation of spermidine or homospermidine from deoxyhypusine by deoxyhypusine synthase. | Park, JH., et al. 2003. J Biol Chem. 278: 32683-91. PMID: 12788913
- A new crystal structure of deoxyhypusine synthase reveals the configuration of the active enzyme and of an enzyme.NAD.inhibitor ternary complex. | Umland, TC., et al. 2004. J Biol Chem. 279: 28697-705. PMID: 15100216
- Deoxyhypusine synthase haploinsufficiency attenuates acute cytokine signaling. | Templin, AT., et al. 2011. Cell Cycle. 10: 1043-9. PMID: 21389784
- Linker-Region Modified Derivatives of the Deoxyhypusine Synthase Inhibitor CNI-1493 Suppress HIV-1 Replication. | Schröder, M., et al. 2016. Arch Pharm (Weinheim). 349: 91-103. PMID: 26725082
- Trypanosomatid Deoxyhypusine Synthase Activity Is Dependent on Shared Active-Site Complementation between Pseudoenzyme Paralogs. | Afanador, GA., et al. 2018. Structure. 26: 1499-1512.e5. PMID: 30197036
- Recessive Rare Variants in Deoxyhypusine Synthase, an Enzyme Involved in the Synthesis of Hypusine, Are Associated with a Neurodevelopmental Disorder. | Ganapathi, M., et al. 2019. Am J Hum Genet. 104: 287-298. PMID: 30661771
- Flexible NAD+ Binding in Deoxyhypusine Synthase Reflects the Dynamic Hypusine Modification of Translation Factor IF5A. | Chen, M., et al. 2020. Int J Mol Sci. 21: PMID: 32752130
- New Series of Potent Allosteric Inhibitors of Deoxyhypusine Synthase. | Tanaka, Y., et al. 2020. ACS Med Chem Lett. 11: 1645-1652. PMID: 34345355
- Deoxyhypusine synthase assay based on the use of polyhistidine-tagged substrate and metal chelate-affinity chromatography. | Tao, Y., et al. 1994. Anal Biochem. 221: 103-8. PMID: 7985780
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DHS Antibody (G-12) | sc-365076 | 200 µg/ml | $322.00 | |||
DHS Antibody (G-12): m-IgG Fc BP-HRP Bundle | sc-537772 | 200 µg Ab; 10 µg BP | $361.00 | |||
DHS Antibody (G-12): m-IgGκ BP-HRP Bundle | sc-535146 | 200 µg Ab; 40 µg BP | $361.00 | |||
DHS Antibody (G-12): m-IgG2a BP-HRP Bundle | sc-548117 | 200 µg Ab; 10 µg BP | $361.00 |