DHS Antibody (F-10): sc-271871
- DHS Antibody (F-10) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 70-369 mapping at the C-terminus of DHS of human origin
- recommended for detection of DHS of human origin by WB, IP, IF and ELISA
- m-IgG1 BP-HRP is the preferred secondary detection reagent for DHS Antibody (F-10) for WB applications. This reagent is now offered in a bundle with DHS Antibody (F-10) (see ordering information below).
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DHS Antibody (F-10) is a mouse monoclonal IgG1 antibody that detects deoxyhypusine synthase (DHS) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DHS plays a pivotal role in the post-translational modification of eukaryotic initiation factor 5A (eIF-5A) by facilitating the formation of hypusine, a unique modification that is critical for eIF-5A′s function in promoting cell proliferation and survival. The importance of hypusine lies in its influence on the stability and activity of eIF-5A, which is essential for various cellular processes, including translation and cell cycle progression. By catalyzing the NAD-dependent oxidative cleavage of spermidine, DHS initiates the hypusine biosynthesis pathway, ultimately leading to the modification of eIF-5A that is necessary for biological activity. Anti-DHS antibody (F-10) serves as an invaluable tool for researchers studying the mechanisms of cell growth and the role of hypusine in cellular functions, providing insights into potential therapeutic targets for diseases characterized by dysregulated cell proliferation.
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DHS Antibody (F-10) References:
- Deoxyhypusine synthase promotes differentiation and proliferation of T helper type 1 (Th1) cells in autoimmune diabetes. | Colvin, SC., et al. 2013. J Biol Chem. 288: 36226-35. PMID: 24196968
- A new non-radioactive deoxyhypusine synthase assay adaptable to high throughput screening. | Park, MH., et al. 2017. Amino Acids. 49: 1793-1804. PMID: 28819816
- Validation of Plasmodium falciparum deoxyhypusine synthase as an antimalarial target. | Aroonsri, A., et al. 2019. PeerJ. 7: e6713. PMID: 31024761
- Discovery of Novel Allosteric Inhibitors of Deoxyhypusine Synthase. | Tanaka, Y., et al. 2020. J Med Chem. 63: 3215-3226. PMID: 32142284
- Development of an activity assay for characterizing deoxyhypusine synthase and its diverse reaction products. | Kaltenegger, E., et al. 2021. FEBS Open Bio. 11: 10-25. PMID: 33247548
- Deoxyhypusine synthase, an essential enzyme for hypusine biosynthesis, is required for proper exocrine pancreas development. | Padgett, LR., et al. 2021. FASEB J. 35: e21473. PMID: 33811703
- Deoxyhypusine synthase promotes a pro-inflammatory macrophage phenotype. | Anderson-Baucum, E., et al. 2021. Cell Metab. 33: 1883-1893.e7. PMID: 34496231
- Investigation of an Allosteric Deoxyhypusine Synthase Inhibitor in P. falciparum. | Aroonsri, A., et al. 2022. Molecules. 27: PMID: 35458660
- Deoxyhypusine synthase is required for the translational regulation of pancreatic beta cell maturation. | Connors, CT., et al. 2023. bioRxiv.. PMID: 37162889
- Novel deoxyhypusine synthase (DHPS) inhibitors target hypusination-induced vasculogenic mimicry (VM) against malignant melanoma. | Zhao, XH., et al. 2024. Pharmacol Res. 209: 107453. PMID: 39393437
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DHS Antibody (F-10) | sc-271871 | 200 µg/ml | $322.00 | |||
DHS Antibody (F-10): m-IgG1 BP-HRP Bundle | sc-542683 | 200 µg Ab; 20 µg BP | $361.00 |