DHS Antibody (D-11): sc-376798
- DHS Antibody (D-11) is a mouse monoclonal IgG3 provided at 200 µg/ml
- specific for an epitope mapping between amino acids 37-71 near the N-terminus of DHS of human origin
- recommended for detection of DHS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and porcine
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for DHS Antibody (D-11). This work is in progress.
QUICK LINKS
SEE ALSO...
DHS Antibody (D-11) is a mouse monoclonal IgG3 antibody that detects deoxyhypusine synthase (DHS) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). DHS plays a pivotal role in the post-translational modification of eukaryotic initiation factor 5A (eIF-5A) by catalyzing the formation of hypusine, a unique modification that is critical for eIF-5A′s function in promoting cell proliferation and regulating cellular processes such as translation and apoptosis. The importance of hypusine lies in its ability to enhance the stability and activity of eIF-5A, which is essential for the proper functioning of ribosomes and the synthesis of proteins necessary for cell growth and division. By facilitating the conversion of spermidine into deoxyhypusine, DHS (D-11) antibody targets a key player in the hypusine biosynthesis pathway, making DHS essential to cellular metabolism and signaling. Anti-DHS antibody (D-11) serves as an invaluable tool for researchers studying the mechanisms of cell proliferation and the role of hypusine in various biological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DHS Antibody (D-11) References:
- Human deoxyhypusine synthase: interrelationship between binding of NAD and substrates. | Lee, CH. and Park, MH. 2000. Biochem J. 352 Pt 3: 851-7. PMID: 11104695
- Screening assay for the identification of deoxyhypusine synthase inhibitors. | Sommer, MN., et al. 2004. J Biomol Screen. 9: 434-8. PMID: 15296643
- Identification of cellular deoxyhypusine synthase as a novel target for antiretroviral therapy. | Hauber, I., et al. 2005. J Clin Invest. 115: 76-85. PMID: 15630446
- Inhibition of deoxyhypusine synthase enhances islet {beta} cell function and survival in the setting of endoplasmic reticulum stress and type 2 diabetes. | Robbins, RD., et al. 2010. J Biol Chem. 285: 39943-52. PMID: 20956533
- The deoxyhypusine synthase mutant dys1-1 reveals the association of eIF5A and Asc1 with cell wall integrity. | Galvão, FC., et al. 2013. PLoS One. 8: e60140. PMID: 23573236
- In silico design, synthesis, and screening of novel deoxyhypusine synthase inhibitors targeting HIV-1 replication. | Schroeder, M., et al. 2014. ChemMedChem. 9: 940-52. PMID: 24616161
- Homospermidine biosynthesis in the cyanobacterium Anabaena requires a deoxyhypusine synthase homologue and is essential for normal diazotrophic growth. | Burnat, M., et al. 2018. Mol Microbiol. 109: 763-780. PMID: 29923645
- The gene coding for human deoxyhypusine synthase (DHPS) maps to chromosome 19p13.11-p13.12. | Jones, T., et al. 1996. Genomics. 35: 635-7. PMID: 8812510
- Enzyme-substrate intermediate at a specific lysine residue is required for deoxyhypusine synthesis. The role of Lys329 in human deoxyhypusine synthase. | Joe, YA., et al. 1997. J Biol Chem. 272: 32679-85. PMID: 9405486
- Branched-chain and unsaturated 1,7-diaminoheptane derivatives as deoxyhypusine synthase inhibitors. | Lee, YB. and Folk, JE. 1998. Bioorg Med Chem. 6: 253-70. PMID: 9568280
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DHS Antibody (D-11) | sc-376798 | 200 µg/ml | $322.00 | |||
DHS (D-11) Neutralizing Peptide | sc-376798 P | 100 µg/0.5 ml | $69.00 |