DGS8 Antibody (H-6): sc-271259

DGS8 Antibody (H-6) is a mouse monoclonal IgG1 antibody that detects DGS8 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DGS8, also known as DiGeorge syndrome critical region 8 protein, is crucial in the etiology of velocardiofacial/DiGeorge syndrome (VCFS/DGS), a condition characterized by developmental anomalies including structural and functional palate defects, conotruncal cardiac malformations, immunodeficiency, and hypocalcemia. DGS8 is encoded by the DGCR8 gene, which is often deleted in individuals with DiGeorge syndrome, leading to a significant role in the disorder. DGS8 is primarily localized in the nucleus, where DGS8 is involved in the biogenesis of microRNAs, essential for regulating gene expression and cellular processes. This nuclear localization underscores DGS8′s function in post-transcriptional regulation, influencing various developmental pathways and cellular responses. Anti-DGS8 antibody (H-6) provides a reliable tool for researchers studying DGS8′s implications in developmental disorders and potential interactions with other proteins involved in microRNA processing, contributing to a deeper understanding of the molecular mechanisms underlying DiGeorge syndrome.

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DGS8 Antibody (H-6) References:

1. Expression levels of the microRNA maturing microprocessor complex component DGCR8 and the RNA-induced silencing complex (RISC) components argonaute-1, argonaute-2, PACT, TARBP1, and TARBP2 in epithelial skin cancer.  |  Sand, M., et al. 2012. Mol Carcinog. 51: 916-22. PMID: 22025453
2. miRNA biogenesis: biological impact in the development of cancer.  |  Romero-Cordoba, SL., et al. 2014. Cancer Biol Ther. 15: 1444-55. PMID: 25482951
3. The kinase ABL phosphorylates the microprocessor subunit DGCR8 to stimulate primary microRNA processing in response to DNA damage.  |  Tu, CC., et al. 2015. Sci Signal. 8: ra64. PMID: 26126715
4. A heterotrimer model of the complete Microprocessor complex revealed by single-molecule subunit counting.  |  Herbert, KM., et al. 2016. RNA. 22: 175-83. PMID: 26683315
5. DGCR8 Acts as an Adaptor for the Exosome Complex to Degrade Double-Stranded Structured RNAs.  |  Macias, S., et al. 2015. Mol Cell. 60: 873-85. PMID: 26687677
6. Structural Basis for pri-miRNA Recognition by Drosha.  |  Jin, W., et al. 2020. Mol Cell. 78: 423-433.e5. PMID: 32220645
7. Bulges control pri-miRNA processing in a position and strand-dependent manner.  |  Li, S., et al. 2021. RNA Biol. 18: 1716-1726. PMID: 33382955
8. The orf virus (ORFV) protein OV20.0 interacts with the microprocessor complex subunit DGCR8 to regulate miRNA biogenesis and ORFV infection.  |  Liao, GR., et al. 2021. FEBS Lett. 595: 2897-2908. PMID: 34778960
9. DGCR8 Microprocessor Subunit Mutation and Expression Deregulation in Thyroid Lesions.  |  Rodrigues, L., et al. 2022. Int J Mol Sci. 23: PMID: 36499151
10. Small nucleolar RNA host gene 18 controls vascular smooth muscle cell contractile phenotype and neointimal hyperplasia.  |  Niu, K., et al. 2024. Cardiovasc Res. 120: 796-810. PMID: 38498586

The preferred DGS8 antibody is DGS8 Antibody (E-10): sc-377249.