DGCR2 CRISPR/Cas9 KO Plasmid (m): sc-419995
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DGCR2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DGCR2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DGCR2 CRISPR/Cas9 KO Plasmid (m) | sc-419995 | 20 µg | $397.00 |
Overview
Dgcr2 encodes DGCR2, a transmembrane protein enriched in developing neural tissues and implicated in processes that support neuronal differentiation, migration, and circuit formation. DGCR2 is located within the 22q11.2 syntenic region and is studied in the context of copy-number variation that influences neurodevelopmental phenotypes and synaptic function. In mouse models, Dgcr2 perturbation is used to probe how altered cell–cell signaling and membrane-associated scaffolding contribute to developmental trajectories in the brain. These features make DGCR2 relevant for mechanistic studies of pathways underlying neurodevelopmental vulnerability and systems-level changes in neural connectivity.
DGCR2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dgcr2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dgcr2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dgcr2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DGCR2 protein expression.
This CRISPR knockout system enables efficient generation of Dgcr2-deficient cell models for investigation of DGCR2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dgcr2 exon(s) critical for DGCR2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dgcr2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DGCR2 CRISPR/Cas9 KO Plasmid (m) and DGCR2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dgcr2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DGCR2 HDR Plasmid (m) and DGCR2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dgcr2 homology arms to support homology-directed repair at defined Dgcr2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.