Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DGAT1 Double Nickase Plasmid (h): sc-401735-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DGAT1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DGAT1 Double Nickase Plasmid (h) and DGAT1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DGAT1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DGAT1 Antibody (A-5): sc-271934
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DGAT1 Double Nickase Plasmid (h) | sc-401735-NIC | 20 µg | $410.00 | |||
DGAT1 Double Nickase Plasmid (h2) | sc-401735-NIC-2 | 20 µg | $410.00 |
DGAT1 encodes diacylglycerol O-acyltransferase 1, an endoplasmic reticulum–associated enzyme that catalyzes the terminal step of triacylglycerol synthesis from diacylglycerol and acyl-CoA. By controlling neutral lipid formation and lipid droplet biogenesis, DGAT1 influences cellular energy storage, lipotoxicity responses, and membrane lipid homeostasis, interfacing with fatty acid metabolism and glycerolipid pathways. DGAT1 activity is relevant to studies of metabolic regulation in adipocytes, hepatocytes, and intestinal epithelium, where triacylglycerol handling shapes nutrient absorption and lipid signaling. Altered DGAT1 expression or function has been investigated in contexts such as dyslipidemia, insulin resistance, hepatic steatosis, and lipid-driven inflammation.
DGAT1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DGAT1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DGAT1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DGAT1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DGAT1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.