DGAT1 Antibody (A-5): sc-271934

DGAT1 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects DGAT1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-DGAT1 antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DGAT1, also known as acyl-coenzyme A:diacylglycerol acyltransferase, plays a crucial role in lipid metabolism by catalyzing the final and committed step in triacylglycerol synthesis, utilizing diacylglycerol and fatty acyl CoA as substrates. This enzymatic activity is vital for converting excess carbohydrates into triglycerides, which are essential for energy storage and metabolic homeostasis. DGAT1 is predominantly located in the endoplasmic reticulum of cells, where DGAT1 facilitates triglyceride assembly, thereby influencing processes such as intestinal fat absorption, lipoprotein assembly, and adipose tissue formation. DGAT1 expression is significantly higher in adipose tissue, underscoring DGAT1′s importance in maintaining energy balance and preventing obesity. Mice deficient in DGAT1 exhibit increased energy expenditure and resistance to obesity, while those lacking both copies of the gene are unable to secrete milk due to impaired triglyceride synthesis in the mammary gland, highlighting DGAT1′s critical role in both metabolic regulation and lactation.

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DGAT1 Antibody (A-5) References:

1. Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content.  |  Winter, A., et al. 2002. Proc Natl Acad Sci U S A. 99: 9300-5. PMID: 12077321
2. Analysis of energy expenditure at different ambient temperatures in mice lacking DGAT1.  |  Chen, HC., et al. 2003. Am J Physiol Endocrinol Metab. 284: E213-8. PMID: 12388146
3. Concerted elevation of acyl-coenzyme A:diacylglycerol acyltransferase (DGAT) activity through independent stimulation of mRNA expression of DGAT1 and DGAT2 by carbohydrate and insulin.  |  Meegalla, RL., et al. 2002. Biochem Biophys Res Commun. 298: 317-23. PMID: 12413942
4. DGAT1-Dependent Lipid Droplet Biogenesis Protects Mitochondrial Function during Starvation-Induced Autophagy.  |  Nguyen, TB., et al. 2017. Dev Cell. 42: 9-21.e5. PMID: 28697336
5. Targeting DGAT1 Ameliorates Glioblastoma by Increasing Fat Catabolism and Oxidative Stress.  |  Cheng, X., et al. 2020. Cell Metab. 32: 229-242.e8. PMID: 32559414
6. Hypoxia-inducible lipid droplet-associated induces DGAT1 and promotes lipid storage in hepatocytes.  |  de la Rosa Rodriguez, MA., et al. 2021. Mol Metab. 47: 101168. PMID: 33465519
7. An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma.  |  Zhou, J., et al. 2022. Mol Cell. 82: 3030-3044.e8. PMID: 35764091
8. Chenodeoxycholic acid suppresses AML progression through promoting lipid peroxidation via ROS/p38 MAPK/DGAT1 pathway and inhibiting M2 macrophage polarization.  |  Liu, J., et al. 2022. Redox Biol. 56: 102452. PMID: 36084349
9. DGKB mediates radioresistance by regulating DGAT1-dependent lipotoxicity in glioblastoma.  |  Kang, H., et al. 2023. Cell Rep Med. 4: 100880. PMID: 36603576
10. Preferential lipolysis of DGAT1 over DGAT2 generated triacylglycerol in Huh7 hepatocytes.  |  Selvaraj, R., et al. 2023. Biochim Biophys Acta Mol Cell Biol Lipids. 1868: 159376. PMID: 37516308
11. Targeting DGAT1 inhibits prostate cancer cells growth by inducing autophagy flux blockage via oxidative stress.  |  Cui, H., et al. 2024. Oncogene. 43: 136-150. PMID: 37973951
12. Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis.  |  Cases, S., et al. 1998. Proc Natl Acad Sci U S A. 95: 13018-23. PMID: 9789033

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DGAT1 Antibody (A-5) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-DGAT1 Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DGAT1 Antibody (A-5) Specifications

Alternate Product Name: DGAT1

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-100

Epitope Target: DGAT1

Epitope Species: human

Product Reactivity/Detection: DGAT1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for DGAT1 Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin