DET1 Antibody (F-2): sc-514348

DET1 Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects DET1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DET1 Antibody (F-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DET1, or de-etiolated homolog 1, is a highly conserved nuclear protein consisting of 550 amino acids, predominantly expressed in the ovary, certain lymphoid organs, and resting leukocytes. DET1 plays a crucial role in the regulation of gene expression and is a key component of the E3 ubiquitin ligase Doublecortin DET1-COP1 complex, which is essential for the ubiquitination and subsequent degradation of the proto-oncogenic transcription factor c-Jun. Notably, DET1 enhances the activity of several members of the ubiquitin-conjugating enzyme family and modulates the functions of Cul-4A, thereby influencing various cellular processes. DET1′s importance extends to its involvement in multiple plant developmental processes, where DET1 acts as a vital negative regulator of photomorphogenesis, ensuring that plants adapt appropriately to light conditions.

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DET1 Antibody (F-2) References:

1. The photomorphogenesis regulator DET1 binds the amino-terminal tail of histone H2B in a nucleosome context.  |  Benvenuto, G., et al. 2002. Curr Biol. 12: 1529-34. PMID: 12225670
2. Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase.  |  Wertz, IE., et al. 2004. Science. 303: 1371-4. PMID: 14739464
3. Manipulation of DET1 expression in tomato results in photomorphogenic phenotypes caused by post-transcriptional gene silencing.  |  Davuluri, GR., et al. 2004. Plant J. 40: 344-54. PMID: 15469492
4. DDB2, DDB1A and DET1 exhibit complex interactions during Arabidopsis development.  |  Al Khateeb, WM. and Schroeder, DF. 2007. Genetics. 176: 231-42. PMID: 17409070
5. Mammalian DET1 regulates Cul4A activity and forms stable complexes with E2 ubiquitin-conjugating enzymes.  |  Pick, E., et al. 2007. Mol Cell Biol. 27: 4708-19. PMID: 17452440
6. Transcriptional repression of c-Jun's E3 ubiquitin ligases contributes to c-Jun induction by UV.  |  Anzi, S., et al. 2008. Cell Signal. 20: 862-71. PMID: 18295447
7. Arabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 forms a nuclear E3 ubiquitin ligase with DDB1 and CUL4 that is involved in multiple plant developmental processes.  |  Zhang, Y., et al. 2008. Plant Cell. 20: 1437-55. PMID: 18552200
8. Effect of Arabidopsis COP10 ubiquitin E2 enhancement activity across E2 families and functional conservation among its canonical homologues.  |  Lau, OS. and Deng, XW. 2009. Biochem J. 418: 683-90. PMID: 19061479
9. The photomorphogenic repressors COP1 and DET1: 20 years later.  |  Lau, OS. and Deng, XW. 2012. Trends Plant Sci. 17: 584-93. PMID: 22705257
10. DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis.  |  Nassrallah, A., et al. 2018. Elife. 7: PMID: 30192741
11. DET1 regulates HY5 through COP1: A new paradigm in the regulation of HY5.  |  Das, S., et al. 2021. Mol Plant. 14: 864-866. PMID: 34048951
12. DET1, a negative regulator of light-mediated development and gene expression in arabidopsis, encodes a novel nuclear-localized protein.  |  Pepper, A., et al. 1994. Cell. 78: 109-16. PMID: 8033202

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DET1 Antibody (F-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-DET1 Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DET1 Antibody (F-2) Specifications

Alternate Product Name: DET1

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: DET1

Epitope Species: human

Product Reactivity/Detection: DET1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for DET1 Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin