Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Desmin CRISPR/Cas9 KO Plasmid (h): sc-400248
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Desmin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Desmin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Desmin Antibody (RD301): sc-23879
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Desmin CRISPR/Cas9 KO Plasmid (h) | sc-400248 | 20 µg | $397.00 |
Overview
DES encodes desmin, a type III intermediate filament protein that forms an essential cytoskeletal scaffold in cardiac, skeletal, and smooth muscle cells. Desmin integrates with Z-disc structures and connects myofibrils to mitochondria, nuclei, and the sarcolemma, supporting mechanotransduction, organelle positioning, and contractile integrity. It participates in cytoskeletal organization and stress-response processes that influence muscle cell stability and remodeling. Disruption of DES is linked to desmin-related myopathies and cardiomyopathies characterized by protein aggregation and progressive muscle dysfunction, making it relevant for studies of proteostasis and muscle degeneration mechanisms.
Desmin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DES gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DES together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DES open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Desmin protein expression.
This CRISPR knockout system enables efficient generation of DES-deficient cell models for investigation of Desmin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DES exon(s) critical for Desmin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DES genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Desmin CRISPR/Cas9 KO Plasmid (h) and Desmin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DES locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Desmin HDR Plasmid (h) and Desmin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DES homology arms to support homology-directed repair at defined DES target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.