Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DEPTOR CRISPR Activation Plasmid (m): sc-430194-ACT
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DEPTOR CRISPR Activation Plasmid (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- DEPTOR CRISPR Activation Plasmid (m) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by DEPTOR CRISPR Activation Plasmid (m) and DEPTOR CRISPR Activation Plasmid (m2) target distinct regulatory regions upstream of the Deptor transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DEPTOR Antibody (A-3): sc-398169
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DEPTOR CRISPR Activation Plasmid (m) | sc-430194-ACT | 20 µg | $397.00 | |||
DEPTOR CRISPR Activation Plasmid (m2) | sc-430194-ACT-2 | 20 µg | $397.00 |
Deptor encodes DEPTOR, a DEP domain–containing scaffold that binds and modulates mTOR complexes, influencing both mTORC1 and mTORC2 signaling outputs. Through its impact on nutrient sensing, protein synthesis, autophagy, and cytoskeletal regulation, DEPTOR helps tune cellular growth and metabolic adaptation. Altered DEPTOR expression has been linked to dysregulated mTOR pathway activity in contexts such as cancer biology, insulin signaling, and immune cell function, making it a useful node for studying pathway feedback and stress responses. In mouse models, Deptor provides a tractable entry point to interrogate how mTOR network wiring shapes proliferation, differentiation, and tissue homeostasis.
DEPTOR CRISPR Activation Plasmid (m) provides a targeted, non-destructive approach to upregulating endogenous Deptor expression without altering the underlying DNA sequence.
DEPTOR CRISPR Activation Plasmid (m) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the Deptor locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the Deptor transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous DEPTOR expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native Deptor locus and enabling the study of DEPTOR-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of DEPTOR pathway restoration in tumor cells with silenced or reduced Deptor expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.