DENND4A CRISPR/Cas9 KO Plasmid (h): sc-407301
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DENND4A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DENND4A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DENND4A CRISPR/Cas9 KO Plasmid (h) | sc-407301 | 20 µg | $397.00 |
Overview
DENND4A encodes a DENN domain–containing protein that functions as a guanine nucleotide exchange factor for Rab GTPases, supporting endosomal trafficking and membrane recycling. Through regulation of Rab-dependent vesicle transport, DENND4A contributes to cargo sorting, receptor turnover, and the spatial organization of signaling complexes that couple membrane dynamics to downstream pathways. Its activity is linked to processes such as nutrient sensing, cytoskeletal remodeling, and compartmentalized signaling on endomembranes, making it relevant for studies of cell growth and stress adaptation. Dysregulation of vesicle trafficking and Rab network control is frequently associated with oncogenic signaling, metabolic imbalance, and neurobiology-related phenotypes, positioning DENND4A as a useful node for dissecting trafficking-associated disease mechanisms.
DENND4A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DENND4A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DENND4A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DENND4A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DENND4A protein expression.
This CRISPR knockout system enables efficient generation of DENND4A-deficient cell models for investigation of DENND4A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DENND4A exon(s) critical for DENND4A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DENND4A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DENND4A CRISPR/Cas9 KO Plasmid (h) and DENND4A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DENND4A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DENND4A HDR Plasmid (h) and DENND4A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DENND4A homology arms to support homology-directed repair at defined DENND4A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.