Delta-3 CRISPR/Cas9 KO Plasmid (m): sc-420011
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Delta-3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Delta-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Delta-3 CRISPR/Cas9 KO Plasmid (m) | sc-420011 | 20 µg | $397.00 |
Overview
Dll3 encodes Delta-3, an atypical Notch ligand that modulates Notch receptor signaling during embryonic development, particularly in somitogenesis and anterior–posterior patterning. In mouse, DLL3 functions primarily within the Golgi and endoplasmic reticulum to influence Notch pathway output by affecting receptor processing and ligand interactions, shaping transcriptional programs that govern cell fate decisions. Perturbation of Dll3 disrupts oscillatory Notch signaling and segmentation clock dynamics, leading to vertebral and rib patterning defects and providing a model for congenital spine malformations. Because Notch signaling also intersects with differentiation, proliferation, and tissue homeostasis, Dll3 loss-of-function studies are useful for dissecting pathway crosstalk in developmental and disease-relevant contexts.
Delta-3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dll3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dll3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dll3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Delta-3 protein expression.
This CRISPR knockout system enables efficient generation of Dll3-deficient cell models for investigation of Delta-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dll3 exon(s) critical for Delta-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dll3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Delta-3 CRISPR/Cas9 KO Plasmid (m) and Delta-3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dll3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Delta-3 HDR Plasmid (m) and Delta-3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dll3 homology arms to support homology-directed repair at defined Dll3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.