DEDD Antibody (H-4): sc-271192
- DEDD Antibody (H-4) is a mouse monoclonal IgG1 κ DEDD antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 278-313 near the C-terminus of DEDD of human origin
- DEDD Antibody (H-4) is recommended for detection of DEDD of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-DEDD Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for DEDD Antibody (H-4) for WB and IHC(P) applications. These reagents are now offered in bundles with DEDD Antibody (H-4) (see ordering information below).
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DEDD Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects DEDD protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DEDD (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DEDD protein, known as Death Effector Domain-containing DNA binding protein, plays a crucial role in apoptosis, a vital physiological process for eliminating unwanted cells in multicellular organisms. DEDD is primarily located in the cytoplasm but translocates to the nucleus during CD95-mediated apoptosis, where DEDD localizes to nucleoli-like structures. This nuclear localization enables DEDD to activate caspase-6 and inhibit RNA polymerase I-dependent transcription, thereby contributing to the regulation of cell death. The N-terminal death effector domain (DED) of DEDD is essential for DNA binding, caspase-6 activation, and specific transcriptional repression of RNA polymerase I, highlighting unique functional properties compared to classical DEDs found in proteins like FADD or caspase-8. DEDD is widely expressed across various tissues, with highest expression levels observed in the testis, and human DEDD gene is mapped to chromosome 1q23.3, with alternative splicing resulting in two transcript variants that encode the same protein.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DEDD Antibody (H-4) References:
- Nuclear localization of DEDD leads to caspase-6 activation through its death effector domain and inhibition of RNA polymerase I dependent transcription. | Schickling, O., et al. 2001. Cell Death Differ. 8: 1157-68. PMID: 11753564
- DEDD and DEDD2 associate with caspase-8/10 and signal cell death. | Alcivar, A., et al. 2003. Oncogene. 22: 291-7. PMID: 12527898
- DEDD, a novel death effector domain-containing protein, targeted to the nucleolus. | Stegh, AH., et al. 1998. EMBO J. 17: 5974-86. PMID: 9774341
- DEFT, a novel death effector domain-containing molecule predominantly expressed in testicular germ cells. | Leo, CP., et al. 1998. Endocrinology. 139: 4839-48. PMID: 9832420
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DEDD Antibody (H-4) | sc-271192 | 200 µg/ml | $322.00 | |||
DEDD Antibody (H-4): m-IgG Fc BP-HRP Bundle | sc-527218 | 200 µg Ab; 10 µg BP | $361.00 | |||
DEDD Antibody (H-4): m-IgG1 BP-HRP Bundle | sc-532591 | 200 µg Ab; 20 µg BP | $361.00 | |||
DEDD Antibody (H-4) AC | sc-271192 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DEDD Antibody (H-4) HRP | sc-271192 HRP | 200 µg/ml | $322.00 | |||
DEDD Antibody (H-4) FITC | sc-271192 FITC | 200 µg/ml | $336.00 | |||
DEDD Antibody (H-4) PE | sc-271192 PE | 200 µg/ml | $349.00 | |||
DEDD Antibody (H-4) Alexa Fluor® 488 | sc-271192 AF488 | 200 µg/ml | $364.00 | |||
DEDD Antibody (H-4) Alexa Fluor® 546 | sc-271192 AF546 | 200 µg/ml | $364.00 | |||
DEDD Antibody (H-4) Alexa Fluor® 594 | sc-271192 AF594 | 200 µg/ml | $364.00 | |||
DEDD Antibody (H-4) Alexa Fluor® 647 | sc-271192 AF647 | 200 µg/ml | $364.00 | |||
DEDD Antibody (H-4) Alexa Fluor® 680 | sc-271192 AF680 | 200 µg/ml | $364.00 | |||
DEDD Antibody (H-4) Alexa Fluor® 790 | sc-271192 AF790 | 200 µg/ml | $364.00 | |||
DEDD (H-4) Neutralizing Peptide | sc-271192 P | 100 µg/0.5 ml | $69.00 |