DDX46 Antibody (B-6): sc-514071
- DDX46 Antibody (B-6) is a mouse monoclonal IgG1 κ DDX46 antibody provided at 200 µg/ml
- raised against amino acids 713-916 mapping within an internal region of DDX46 of human origin
- DDX46 Antibody (B-6) is recommended for detection of DDX46 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DDX46 Antibody (B-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for DDX46 Antibody (B-6) for WB applications. These reagents are now offered in bundles with DDX46 Antibody (B-6) (see ordering information below).
DDX46 Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects DDX46 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DDX46 Antibody (B-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DDX46, also known as DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 or PRPF5, is a 1,031 amino acid protein that plays a pivotal role in pre-mRNA splicing as an integral component of the 17S U2 small nuclear ribonucleoprotein (snRNP). DDX46 is primarily localized to nuclear speckles, which are essential for the assembly and modification of RNA-protein complexes involved in splicing. DDX46′s presence in these nuclear domains highlights its importance in regulating gene expression and ensuring proper RNA processing, which is crucial for cellular function and development. Additionally, DDX46 is characterized by its helicase C-terminal domain and helicase ATP-binding domain, which are vital for RNA unwinding activity, further underscoring DDX46′s role in facilitating the dynamic changes in RNA structure necessary for effective splicing and ribonucleoprotein complex formation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DDX46 Antibody (B-6) References:
- Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein. | Will, CL., et al. 2002. EMBO J. 21: 4978-88. PMID: 12234937
- The human DDX and DHX gene families of putative RNA helicases. | Abdelhaleem, M., et al. 2003. Genomics. 81: 618-22. PMID: 12782131
- Prp5 bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA. | Xu, YZ., et al. 2004. EMBO J. 23: 376-85. PMID: 14713954
- The newly discovered Q motif of DEAD-box RNA helicases regulates RNA-binding and helicase activity. | Cordin, O., et al. 2004. EMBO J. 23: 2478-87. PMID: 15201868
- D-E-A-D protein family of putative RNA helicases. | Schmid, SR. and Linder, P. 1992. Mol Microbiol. 6: 283-91. PMID: 1552844
- Nucleolar proteome dynamics. | Andersen, JS., et al. 2005. Nature. 433: 77-83. PMID: 15635413
- Dead-box proteins: a family affair--active and passive players in RNP-remodeling. | Linder, P. 2006. Nucleic Acids Res. 34: 4168-80. PMID: 16936318
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX46 Antibody (B-6) | sc-514071 | 200 µg/ml | $322.00 | |||
DDX46 Antibody (B-6): m-IgG Fc BP-HRP Bundle | sc-527848 | 200 µg Ab; 10 µg BP | $361.00 | |||
DDX46 Antibody (B-6): m-IgG1 BP-HRP Bundle | sc-533221 | 200 µg Ab; 20 µg BP | $361.00 | |||
DDX46 Antibody (B-6) AC | sc-514071 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DDX46 Antibody (B-6) HRP | sc-514071 HRP | 200 µg/ml | $322.00 | |||
DDX46 Antibody (B-6) FITC | sc-514071 FITC | 200 µg/ml | $336.00 | |||
DDX46 Antibody (B-6) PE | sc-514071 PE | 200 µg/ml | $349.00 | |||
DDX46 Antibody (B-6) Alexa Fluor® 488 | sc-514071 AF488 | 200 µg/ml | $364.00 | |||
DDX46 Antibody (B-6) Alexa Fluor® 546 | sc-514071 AF546 | 200 µg/ml | $364.00 | |||
DDX46 Antibody (B-6) Alexa Fluor® 594 | sc-514071 AF594 | 200 µg/ml | $364.00 | |||
DDX46 Antibody (B-6) Alexa Fluor® 647 | sc-514071 AF647 | 200 µg/ml | $364.00 | |||
DDX46 Antibody (B-6) Alexa Fluor® 680 | sc-514071 AF680 | 200 µg/ml | $364.00 | |||
DDX46 Antibody (B-6) Alexa Fluor® 790 | sc-514071 AF790 | 200 µg/ml | $364.00 |