DDX18 CRISPR/Cas9 KO Plasmid (h): sc-406057
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDX18 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DDX18 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX18 CRISPR/Cas9 KO Plasmid (h) | sc-406057 | 20 µg | $397.00 |
Overview
DDX18 encodes a nucleolar DEAD-box RNA helicase that couples ATP-dependent RNA remodeling to ribosome biogenesis and RNA processing. DDX18 participates in pre-rRNA maturation, assembly of preribosomal particles, and coordination of nucleolar stress responses that influence cell-cycle progression and global protein synthesis capacity. Perturbation of DDX18 function can disrupt rRNA processing and translation homeostasis, linking this factor to proliferative control and genome maintenance programs. Altered expression or dependency on DDX18 has been observed in cancer-related transcriptional and functional studies, supporting its use as a mechanistic node in growth-associated RNA metabolism.
DDX18 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DDX18 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DDX18 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DDX18 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DDX18 protein expression.
This CRISPR knockout system enables efficient generation of DDX18-deficient cell models for investigation of DDX18 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DDX18 exon(s) critical for DDX18 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DDX18 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DDX18 CRISPR/Cas9 KO Plasmid (h) and DDX18 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DDX18 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DDX18 HDR Plasmid (h) and DDX18 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DDX18 homology arms to support homology-directed repair at defined DDX18 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.