DDRGK1 CRISPR/Cas9 KO Plasmid (h): sc-413090
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDRGK1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DDRGK1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDRGK1 CRISPR/Cas9 KO Plasmid (h) | sc-413090 | 20 µg | $397.00 |
Overview
DDRGK1 (DDRGK domain containing 1; also known as UFBP1) is an essential component of the UFM1 conjugation system that supports endoplasmic reticulum (ER) homeostasis and protein quality control. The protein participates in UFMylation at the ER, linking ribosome-associated processes and ER-associated degradation to cellular stress adaptation, including the unfolded protein response. Through these pathways, DDRGK1 influences proteostasis, cell survival under ER stress, and coordination of secretory protein handling. Dysregulation of UFM1 pathway factors, including DDRGK1, has been associated with cellular vulnerability in contexts such as hematopoietic dysfunction and stress-related disease biology, making it a useful target for mechanistic studies.
DDRGK1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DDRGK1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DDRGK1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DDRGK1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DDRGK1 protein expression.
This CRISPR knockout system enables efficient generation of DDRGK1-deficient cell models for investigation of DDRGK1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DDRGK1 exon(s) critical for DDRGK1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DDRGK1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DDRGK1 CRISPR/Cas9 KO Plasmid (h) and DDRGK1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DDRGK1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DDRGK1 HDR Plasmid (h) and DDRGK1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DDRGK1 homology arms to support homology-directed repair at defined DDRGK1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.