DDEF1 Antibody (B-10): sc-374410
- DDEF1 Antibody (B-10) is a mouse monoclonal IgG2a κ DDEF1 antibody, cited in 4 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 837-873 near the C-terminus of DDEF1 of human origin
- DDEF1 Antibody (B-10) is recommended for detection of DDEF1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DDEF1 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for DDEF1 Antibody (B-10) for WB applications. This reagent is now offered in a bundle with DDEF1 Antibody (B-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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DDEF1 Antibody (B-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects DDEF1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DDEF1 (B-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DDEF1, also known as development and differentiation enhancing factor 1, ASAP1, AMAP1, or PAG2, functions as an ADP ribosylation factor (ARF)-GTPase activating protein (GAP) that plays a crucial role in regulating cellular signaling pathways. DDEF1 is primarily localized in the cytoplasm and at newly formed focal complexes at the cell periphery, where DDEF1 interacts with key signaling proteins such as ARF1, ARF5, ARF6, and SRK (ZAP-70). These interactions are vital for influencing cell motility and actin cytoskeletal remodeling, as well as for the differentiation of adipocytes and fibroblasts. DDEF1 contains two ankyrin repeats, one ARF-GAP domain, one SH3 domain, and one pleckstrin homology domain, which are essential for the phosphoinositide-dependent regulation of ARFs. Notably, DDEF1 overexpression has been associated with the inhibition of invasion and metastasis in breast cancer and high-grade uveal melanomas, highlighting DDEF1′s potential as a therapeutic target and diagnostic marker for specific cancers.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DDEF1 Antibody (B-10) References:
- DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. | Furman, C., et al. 2002. J Biol Chem. 277: 7962-9. PMID: 11773070
- Expression of AMAP1, an ArfGAP, provides novel targets to inhibit breast cancer invasive activities. | Onodera, Y., et al. 2005. EMBO J. 24: 963-73. PMID: 15719014
- DDEF1 is located in an amplified region of chromosome 8q and is overexpressed in uveal melanoma. | Ehlers, JP., et al. 2005. Clin Cancer Res. 11: 3609-13. PMID: 15897555
- Regulation of ASAP1 by phospholipids is dependent on the interface between the PH and Arf GAP domains. | Che, MM., et al. 2005. Cell Signal. 17: 1276-88. PMID: 16038802
- Mutational analysis of the Arf1*GTP/Arf GAP interface reveals an Arf1 mutant that selectively affects the Arf GAP ASAP1. | Luo, R., et al. 2005. Curr Biol. 15: 2164-9. PMID: 16332543
- A BAR domain in the N terminus of the Arf GAP ASAP1 affects membrane structure and trafficking of epidermal growth factor receptor. | Nie, Z., et al. 2006. Curr Biol. 16: 130-9. PMID: 16431365
- Targeting AMAP1 and cortactin binding bearing an atypical src homology 3/proline interface for prevention of breast cancer invasion and metastasis. | Hashimoto, S., et al. 2006. Proc Natl Acad Sci U S A. 103: 7036-41. PMID: 16636290
- Arf GAPs as regulators of the actin cytoskeleton. | Randazzo, PA., et al. 2007. Biol Cell. 99: 583-600. PMID: 17868031
- Src-dependent phosphorylation of ASAP1 regulates podosomes. | Bharti, S., et al. 2007. Mol Cell Biol. 27: 8271-83. PMID: 17893324
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDEF1 Antibody (B-10) | sc-374410 | 200 µg/ml | $316.00 | |||
DDEF1 Antibody (B-10): m-IgGκ BP-HRP Bundle | sc-522729 | 200 µg Ab, 40 µg BP | $354.00 | |||
DDEF1 Antibody (B-10) AC | sc-374410 AC | 500 µg/ml, 25% agarose | $416.00 | |||
DDEF1 Antibody (B-10) HRP | sc-374410 HRP | 200 µg/ml | $316.00 | |||
DDEF1 Antibody (B-10) FITC | sc-374410 FITC | 200 µg/ml | $330.00 | |||
DDEF1 Antibody (B-10) PE | sc-374410 PE | 200 µg/ml | $343.00 | |||
DDEF1 Antibody (B-10) Alexa Fluor® 488 | sc-374410 AF488 | 200 µg/ml | $357.00 | |||
DDEF1 Antibody (B-10) Alexa Fluor® 546 | sc-374410 AF546 | 200 µg/ml | $357.00 | |||
DDEF1 Antibody (B-10) Alexa Fluor® 594 | sc-374410 AF594 | 200 µg/ml | $357.00 | |||
DDEF1 Antibody (B-10) Alexa Fluor® 647 | sc-374410 AF647 | 200 µg/ml | $357.00 | |||
DDEF1 Antibody (B-10) Alexa Fluor® 680 | sc-374410 AF680 | 200 µg/ml | $357.00 | |||
DDEF1 Antibody (B-10) Alexa Fluor® 790 | sc-374410 AF790 | 200 µg/ml | $357.00 | |||
DDEF1 (B-10) Neutralizing Peptide | sc-374410 P | 100 µg/0.5 ml | $68.00 |