DDC Double Nickase Plasmid (m): sc-419967-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDC Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DDC Double Nickase Plasmid (m) and DDC Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ddc. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDC Antibody (8E8): sc-293287
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDC Double Nickase Plasmid (m) | sc-419967-NIC | 20 µg | $410.00 |
Mouse Ddc encodes aromatic L-amino acid decarboxylase (DDC), a pyridoxal phosphate–dependent enzyme that converts L-DOPA to dopamine and 5-hydroxytryptophan to serotonin, positioning it at a key junction of catecholamine and indoleamine neurotransmitter biosynthesis. DDC activity supports monoaminergic neurotransmission and neuromodulatory signaling that influence neuronal development, synaptic plasticity, and neuroendocrine regulation. In the central and peripheral nervous systems, Ddc expression contributes to metabolic flux through dopamine and serotonin pathways, with downstream effects on vesicular transport, receptor signaling, and oxidative stress balance. Altered DDC function or monoamine availability is relevant to experimental models of movement and affective phenotypes and to studies of neurotransmitter-dependent neurodevelopmental and neurodegenerative processes.
DDC Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ddc locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ddc. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ddc function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ddc-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.