Dcun1D4 CRISPR/Cas9 KO Plasmid (h): sc-409238
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dcun1D4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Dcun1D4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dcun1D4 CRISPR/Cas9 KO Plasmid (h) | sc-409238 | 20 µg | $397.00 |
Overview
DCUN1D4 encodes Dcun1D4, a member of the DCN1-like family implicated in regulation of cullin-RING E3 ubiquitin ligase activity through control of cullin neddylation. By modulating NEDD8-dependent activation of cullin complexes, Dcun1D4 can influence ubiquitin-mediated proteostasis, cell-cycle progression, and signaling pathways that depend on timely substrate turnover. Altered activity within the neddylation–ubiquitin axis is broadly relevant to genome stability and stress-response programs, making DCUN1D4 a useful locus for interrogating pathway specificity across CRL-dependent processes. Dysregulation of ubiquitin and neddylation machinery is observed across multiple disease contexts, supporting mechanistic studies of DCUN1D4 in cellular homeostasis and pathway perturbation.
Dcun1D4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DCUN1D4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DCUN1D4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DCUN1D4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dcun1D4 protein expression.
This CRISPR knockout system enables efficient generation of DCUN1D4-deficient cell models for investigation of Dcun1D4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DCUN1D4 exon(s) critical for Dcun1D4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DCUN1D4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Dcun1D4 CRISPR/Cas9 KO Plasmid (h) and Dcun1D4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DCUN1D4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Dcun1D4 HDR Plasmid (h) and Dcun1D4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DCUN1D4 homology arms to support homology-directed repair at defined DCUN1D4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.