DCTD Antibody (F-9): sc-376659

DCTD Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects DCTD protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DCTD (F-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DCTD, or Deoxycytidylate deaminase, is a crucial allosteric enzyme composed of 178 amino acids that functions as a homohexamer, belonging to the cytidine and deoxycytidylate deaminase protein family. This enzyme plays a vital role in nucleotide metabolism by catalyzing the conversion of deoxycytidine monophosphate (dCMP) to deoxyuridine monophosphate (dUMP), a key substrate for thymidylate synthase (TS). TS subsequently utilizes dUMP along with 5,10-methylenetetrahydrofolate to synthesize deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. The regulation of DCTD activity is influenced by the concentrations of end products, deoxycytidine triphosphate (dCTP) and deoxythymidine triphosphate (dTTP), highlighting DCTD′s importance in maintaining nucleotide balance within the cell. Alternative splicing events lead to the expression of multiple DCTD isoforms, further underscoring the complexity and regulatory mechanisms governing DCTD function in cellular processes.

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DCTD Antibody (F-9) References:

1. Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.  |  Rush, J., et al. 2005. Nat Biotechnol. 23: 94-101. PMID: 15592455
2. Gemcitabine pharmacogenomics: cytidine deaminase and deoxycytidylate deaminase gene resequencing and functional genomics.  |  Gilbert, JA., et al. 2006. Clin Cancer Res. 12: 1794-803. PMID: 16551864
3. Chloroviruses encode a bifunctional dCMP-dCTP deaminase that produces two key intermediates in dTTP formation.  |  Zhang, Y., et al. 2007. J Virol. 81: 7662-71. PMID: 17475641
4. An association between RRM1 haplotype and gemcitabine-induced neutropenia in breast cancer patients.  |  Rha, SY., et al. 2007. Oncologist. 12: 622-30. PMID: 17602053
5. Involvement of deoxycytidylate deaminase in the response to S(n)1-type methylation DNA damage in budding yeast.  |  Liskay, RM., et al. 2007. Curr Biol. 17: R755-7. PMID: 17803923
6. Deoxycytidylate deaminase activity in non-stimulated and phytohemagglutinin-stimulated human lymphocytes, and in leukemic cells.  |  Tyrsted, G., et al. 1987. Mol Cell Biochem. 76: 27-34. PMID: 3627112
7. Chromosomal location and structural organization of the human deoxycytidylate deaminase gene.  |  Weiner, KX., et al. 1995. J Biol Chem. 270: 18727-9. PMID: 7642519
8. Primary structure of human deoxycytidylate deaminase and overexpression of its functional protein in Escherichia coli.  |  Weiner, KX., et al. 1993. J Biol Chem. 268: 12983-9. PMID: 7685356

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DCTD Antibody (F-9) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-DCTD Antibody (F-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DCTD Antibody (F-9) Specifications

Alternate Product Name: DCTD

Product Clone Name: F-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-178

Epitope Target: DCTD

Epitope Species: human

Product Reactivity/Detection: DCTD

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for DCTD Antibody (F-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin