DCAF16 CRISPR/Cas9 KO Plasmid (h): sc-412364
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DCAF16 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DCAF16 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DCAF16 CRISPR/Cas9 KO Plasmid (h) | sc-412364 | 20 µg | $397.00 |
Overview
DCAF16 encodes a DDB1- and CUL4-associated factor implicated in substrate recognition for the CRL4 E3 ubiquitin ligase machinery, linking it to ubiquitin-dependent proteostasis and regulation of nuclear protein turnover. As part of cullin-RING ubiquitination pathways, DCAF16 has been connected to quality control processes that influence transcriptional programs and stress-responsive signaling in the nucleus. Altered ubiquitin ligase adaptor function can perturb cell-cycle control, DNA damage responses, and RNA metabolism, making DCAF16 a relevant node for mechanistic studies in cancer biology and other disorders involving protein homeostasis dysregulation.
DCAF16 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DCAF16 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DCAF16 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DCAF16 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DCAF16 protein expression.
This CRISPR knockout system enables efficient generation of DCAF16-deficient cell models for investigation of DCAF16 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DCAF16 exon(s) critical for DCAF16 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DCAF16 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DCAF16 CRISPR/Cas9 KO Plasmid (h) and DCAF16 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DCAF16 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DCAF16 HDR Plasmid (h) and DCAF16 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DCAF16 homology arms to support homology-directed repair at defined DCAF16 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.