Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DC-SIGN Double Nickase Plasmid (h): sc-401368-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DC-SIGN Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DC-SIGN Double Nickase Plasmid (h) and DC-SIGN Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD209. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DC-SIGN Antibody (DC28): sc-65740
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DC-SIGN Double Nickase Plasmid (h) | sc-401368-NIC | 20 µg | $410.00 | |||
DC-SIGN Double Nickase Plasmid (h2) | sc-401368-NIC-2 | 20 µg | $410.00 |
CD209 encodes DC-SIGN (CD209), a C-type lectin receptor expressed primarily on dendritic cells and certain macrophage populations that binds high-mannose and fucosylated glycans on microbes and endogenous ligands. Through calcium-dependent carbohydrate recognition and signaling via associated adaptors, DC-SIGN modulates antigen uptake, endocytosis, and downstream pathways such as NF-κB and Raf-1–dependent signaling that shape cytokine responses and T cell priming. This receptor contributes to pathogen recognition and immune regulation at mucosal and peripheral tissues, influencing leukocyte trafficking and cell–cell adhesion through interactions with ICAM family members. Altered DC-SIGN activity and expression have been linked to variation in susceptibility to infectious challenge and inflammatory immune phenotypes, making CD209 a relevant target for mechanistic immunology studies.
DC-SIGN Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD209 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD209. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD209 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD209-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.