DC-SIGN Antibody (DC28): sc-65740

DC-SIGN Antibody (DC28) is a mouse monoclonal IgG2a kappa light chain antibody that detects DC-SIGN protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). DC-SIGN (DC28) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DC-SIGN, also known as CD209, is a type II membrane protein predominantly expressed on dendritic cells (DCs), which are crucial antigen-presenting cells in the immune system. These cells are strategically located in peripheral mucosal tissues and migrate to lymphoid organs, playing a vital role in initiating immune responses. DC-SIGN is essential for mediating the interaction between dendritic cells and resting T cells, as DC-SIGN binds to ICAM-3, facilitating the formation of the immunological synapse. This interaction is particularly significant in HIV-1 infection, as dendritic cells capture the virus through DC-SIGN, promoting viral transport from mucosal surfaces to lymphoid tissues, where HIV-1 can efficiently infect T cells. Understanding DC-SIGN function is critical for developing therapeutic strategies against HIV-1 and enhancing immune responses, making anti-DC-SIGN antibody (DC28) an invaluable tool for researchers studying immune interactions and viral pathogenesis.

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DC-SIGN Antibody (DC28) References:

1. DC-SIGN: a guide to some mysteries of dendritic cells.  |  Steinman, RM. 2000. Cell. 100: 491-4. PMID: 10721985
2. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses.  |  Geijtenbeek, TB., et al. 2000. Cell. 100: 575-85. PMID: 10721994
3. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells.  |  Geijtenbeek, TB., et al. 2000. Cell. 100: 587-97. PMID: 10721995
4. AIDS research. Novel protein delivers HIV to target cells.  |  Cohen, J. 2000. Science. 287: 1567. PMID: 10733412
5. Functional evaluation of DC-SIGN monoclonal antibodies reveals DC-SIGN interactions with ICAM-3 do not promote human immunodeficiency virus type 1 transmission.  |  Wu, L., et al. 2002. J Virol. 76: 5905-14. PMID: 12021323
6. Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3)-grabbing nonintegrin (DC-SIGN, CD209), a C-type surface lectin in human DCs, is a receptor for Leishmania amastigotes.  |  Colmenares, M., et al. 2002. J Biol Chem. 277: 36766-9. PMID: 12122001
7. The HIV protease inhibitor indinavir reduces immature dendritic cell transendothelial migration.  |  Whelan, KT., et al. 2003. Eur J Immunol. 33: 2520-30. PMID: 12938228
8. Sequence and expression of a membrane-associated C-type lectin that exhibits CD4-independent binding of human immunodeficiency virus envelope glycoprotein gp120.  |  Curtis, BM., et al. 1992. Proc Natl Acad Sci U S A. 89: 8356-60. PMID: 1518869
9. Hepatitis C virus targets DC-SIGN and L-SIGN to escape lysosomal degradation.  |  Ludwig, IS., et al. 2004. J Virol. 78: 8322-32. PMID: 15254204
10. DC-SIGN modulates DC maturation and function in rat renal tubulointerstitial lesions.  |  Cai, M., et al. 2012. Front Biosci (Landmark Ed). 17: 1795-803. PMID: 22201836
11. A multivalent inhibitor of the DC-SIGN dependent uptake of HIV-1 and Dengue virus.  |  Varga, N., et al. 2014. Biomaterials. 35: 4175-84. PMID: 24508075
12. DC-SIGN binding to mannosylated B-cell receptors in follicular lymphoma down-modulates receptor signaling capacity.  |  Valle-Argos, B., et al. 2021. Sci Rep. 11: 11676. PMID: 34083646

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DC-SIGN Antibody (DC28) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-DC-SIGN Antibody (DC28) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DC-SIGN Antibody (DC28) Specifications

Alternate Product Name: CD209

Product Clone Name: DC28

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: DC-SIGN

Epitope Species: human

Product Reactivity/Detection: DC-SIGN

Species Reactivity/Detection: human

Additional Mammalian Reactivity: pig tailed macaque and rhesus macaque

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for DC-SIGN Antibody (DC28): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin