Dbs Antibody (C-7): sc-376400
- Dbs Antibody (C-7) is a mouse monoclonal IgG1 κ Dbs antibody provided at 200 µg/ml
- raised against amino acids mapping within an internal region of Dbs of human origin
- Dbs Antibody (C-7) is recommended for detection of Dbs of human origin by WB, IP, IF and ELISA
- Anti-Dbs Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Dbs Antibody (C-7) for WB applications. These reagents are now offered in bundles with Dbs Antibody (C-7) (see ordering information below).
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SEE ALSO...
Dbs Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dbs protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Dbs (C-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dbs protein, also known as Ost or MCF2L, plays a crucial role as a guanine nucleotide exchange factor (GEF) for Rho guanosine triphosphatases, specifically RhoA and Cdc42, which are vital for regulating cellular processes such as growth and differentiation. The unique structure of Dbs includes a tandem domain arrangement, featuring a Dbl homology (DH) catalytic domain followed by a pleckstrin homology (PH) regulatory domain, which is essential for its function. Notably, Dbs requires the presence of the PH domain to activate the DH domain′s intrinsic catalytic activity, distinguishing Dbs from other members of the Dbl family. Expression of Dbs is notably high in the brain and low in the thymus and spleen, indicating a significant role in neural signaling pathways. Furthermore, Dbs is involved in multiple signaling cascades, including the activation of transcription factors such as Elk-1, Jun, and NFkB, and stimulates transcription from the cyclin D1 promoter, highlighting importance in cell cycle regulation and potential implications in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Dbs Antibody (C-7) References:
- Dependence of Dbl and Dbs transformation on MEK and NF-kappaB activation. | Whitehead, IP., et al. 1999. Mol Cell Biol. 19: 7759-70. PMID: 10523665
- A crystallographic view of interactions between Dbs and Cdc42: PH domain-assisted guanine nucleotide exchange. | Rossman, KL., et al. 2002. EMBO J. 21: 1315-26. PMID: 11889037
- Multifunctional roles for the PH domain of Dbs in regulating Rho GTPase activation. | Rossman, KL., et al. 2003. J Biol Chem. 278: 18393-400. PMID: 12637522
- Critical role of the pleckstrin homology domain in Dbs signaling and growth regulation. | Fuentes, EJ., et al. 2003. J Biol Chem. 278: 21188-96. PMID: 12637530
- Retroviral transduction and oncogenic selection of a cDNA encoding Dbs, a homolog of the Dbl guanine nucleotide exchange factor. | Whitehead, I., et al. 1995. Oncogene. 10: 713-21. PMID: 7862449
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dbs Antibody (C-7) | sc-376400 | 200 µg/ml | $316.00 | |||
Dbs Antibody (C-7): m-IgGκ BP-HRP Bundle | sc-522941 | 200 µg Ab, 40 µg BP | $354.00 | |||
Dbs Antibody (C-7): m-IgG1 BP-HRP Bundle | sc-533538 | 200 µg Ab; 20 µg BP | $354.00 | |||
Dbs Antibody (C-7) AC | sc-376400 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Dbs Antibody (C-7) HRP | sc-376400 HRP | 200 µg/ml | $316.00 | |||
Dbs Antibody (C-7) FITC | sc-376400 FITC | 200 µg/ml | $330.00 | |||
Dbs Antibody (C-7) PE | sc-376400 PE | 200 µg/ml | $343.00 | |||
Dbs Antibody (C-7) Alexa Fluor® 488 | sc-376400 AF488 | 200 µg/ml | $357.00 | |||
Dbs Antibody (C-7) Alexa Fluor® 546 | sc-376400 AF546 | 200 µg/ml | $357.00 | |||
Dbs Antibody (C-7) Alexa Fluor® 594 | sc-376400 AF594 | 200 µg/ml | $357.00 | |||
Dbs Antibody (C-7) Alexa Fluor® 647 | sc-376400 AF647 | 200 µg/ml | $357.00 | |||
Dbs Antibody (C-7) Alexa Fluor® 680 | sc-376400 AF680 | 200 µg/ml | $357.00 | |||
Dbs Antibody (C-7) Alexa Fluor® 790 | sc-376400 AF790 | 200 µg/ml | $357.00 |