DBR1 Antibody (3A7): sc-517060

DBR1 Antibody (3A7) is a mouse monoclonal IgG2a kappa light chain antibody that detects DBR1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-DBR1 antibody (3A7) is available as the non-conjugated format. DBR1, also known as debranching enzyme homolog 1, is a 544 amino acid protein that plays a crucial role in RNA processing by localizing to the nucleus and belonging to the lariat debranching enzyme family. DBR1 catalyzes the cleavage of the 2′-5′ phosphodiester linkage at the branch point of lariat intron pre-mRNAs, converting these lariat structures into linear molecules that can be further degraded. This enzymatic activity is vital for ribonucleotide turnover and is particularly important in retroviral replication, including HIV-1, highlighting DBR1′s significance in viral pathogenesis and cellular RNA metabolism. Human DBR1 exhibits 79% homology with its mouse counterpart, indicating a conserved functional role across species, and is expressed as two alternatively spliced isoforms encoded by a gene located on human chromosome 3.

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DBR1 Antibody (3A7) References:

1. Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants.  |  Kim, JW., et al. 2000. Nucleic Acids Res. 28: 3666-73. PMID: 10982890
2. Prp43 is an essential RNA-dependent ATPase required for release of lariat-intron from the spliceosome.  |  Martin, A., et al. 2002. J Biol Chem. 277: 17743-50. PMID: 11886864
3. DBR1 siRNA inhibition of HIV-1 replication.  |  Ye, Y., et al. 2005. Retrovirology. 2: 63. PMID: 16232320
4. Structure-function analysis of yeast RNA debranching enzyme (Dbr1), a manganese-dependent phosphodiesterase.  |  Khalid, MF., et al. 2005. Nucleic Acids Res. 33: 6349-60. PMID: 16275784
5. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.  |  Olsen, JV., et al. 2006. Cell. 127: 635-48. PMID: 17081983
6. Ty1 reverse transcriptase does not read through the proposed 2',5'-branched retrotransposition intermediate in vitro.  |  Pratico, ED. and Silverman, SK. 2007. RNA. 13: 1528-36. PMID: 17652136
7. Isolation and characterization of the gene encoding yeast debranching enzyme.  |  Chapman, KB. and Boeke, JD. 1991. Cell. 65: 483-92. PMID: 1850323
8. Purification of a RNA debranching activity from HeLa cells.  |  Arenas, J. and Hurwitz, J. 1987. J Biol Chem. 262: 4274-9. PMID: 2435736

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DBR1 Antibody (3A7) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-DBR1 Antibody (3A7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DBR1 Antibody (3A7) Specifications

Alternate Product Name: DBR1

Product Clone Name: 3A7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 445-538

Epitope Target: DBR1

Epitope Species: human

Product Reactivity/Detection: DBR1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for DBR1 Antibody (3A7): N/A