Dbf4b Antibody (1A7): sc-517110

Dbf4b Antibody (1A7) is a mouse monoclonal IgG1 antibody that detects Dbf4b in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Dbf4b plays a crucial role in the regulation of the cell cycle, particularly during the S and M phases, by forming a complex with Cdc7, which is essential for the activation of replication origins. This complex is vital for the phosphorylation of several key proteins necessary for DNA replication initiation, including the minichromosome maintenance proteins and the p180 subunit of DNA polymerase alpha-primase. Dbf4b, a 615 amino acid nuclear protein, is characterized by the presence of one BRCT domain and one DBF4-type zinc finger, which are important for its interaction with other proteins and regulatory functions. Notably, Dbf4b is widely expressed, with the highest levels found in the testis, and expression increases significantly as cells transition into the S phase, maintaining elevated levels through M phase. This regulation is critical for ensuring proper DNA replication and cell division, highlighting Dbf4b′s importance in cellular proliferation and genomic stability. Anti-Dbf4b antibody (1A7) is an essential tool for researchers studying cell cycle dynamics and the molecular mechanisms underlying DNA replication.

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Dbf4b Antibody (1A7) References:

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